| Field | Specification |
|---|---|
| Mfr No | |
| Detection Range | |
| Product Type | |
| Sample Type(s) | serum, plasma or other biological fluids. |
| Sensitivity | |
| Species |
Assay Principle
Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) is a biomarker protein expressed in mammalian tissues and biological fluids. Dysregulation of NAADP has been implicated in cellular signaling and fundamental cell biology, making it a relevant biomarker for investigational studies.
Quantitative measurement of NAADP enables researchers to characterize its abundance in Serum, Plasma and correlate levels with experimental conditions. Studies investigating NAADP have contributed to the understanding of cell signaling pathways, disease pathogenesis, and therapeutic target validation.
This competitive ELISA kit provides a validated, reproducible format for NAADP quantification within the inhibition range of 0.156-10ng/mL. The assay supports experimental workflows in Cell Biology research.
Kit Components
Each kit contains sufficient reagents for one 96-well plate (approximately 40 samples in duplicate with standard curve):
Pre-coated microplate (96 wells), HRP-conjugated antigen, standard protein, sample diluent, wash buffer, TMB substrate solution, stop solution, and product manual.
Validated LotPerformance Data
| Assay Type | Competitive ELISA |
|---|---|
| Detection Range | 0.156-10ng/mL |
| Sensitivity (LLOD) | 0.055ng/mL |
| Validated Sample Types | Serum, Plasma |
| Detection Method | Colorimetric (450 nm / 540–570 nm reference) |
| Plate Format | 96-well microplate |
| Shelf Life | Traditional: 12 months | Ready-to-use: 16 months |
Assay Procedure Summary
Add samples and HRP-conjugated antigen simultaneously to pre-coated wells and incubate. Wash, add TMB substrate, and read at 450 nm. Signal is inversely proportional to Nicotinic Acid Adenine Dinucleotide Phosphate concentration. Construct a standard inhibition curve from the provided reference antigen to calculate sample concentrations.
Pre-warm all kit components to room temperature (18–25 °C) for 30 minutes before starting. Run all samples in duplicate. Centrifuge samples at ≥1,000 × g before use to remove particulates.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorDLdevelopCatalog No.DL-NAADP-Ge, DLR-NAADP-GeSpeciesSee title
This kit is validated for serum and plasma. Centrifuge samples at ≥1,000 × g for 15 min to remove particulates. Avoid hemolyzed, lipemic, or heat-inactivated samples. As a competitive format, higher analyte concentration produces lower signal — dilute proportionally. Pre-dilute samples within the linear range before committing full plates.
Minimum detectable concentration: 0.055ng/mL. Quantitative standard curve range: 0.156-10ng/mL. Samples above the upper limit must be diluted; near-lower-limit samples require duplicate measurement.
A typical sandwich ELISA protocol for this kit requires 3.5–5 hours. Pre-warm reagents to room temperature (18–25 °C) for at least 30 minutes before starting and prepare standard dilutions while the capture plate equilibrates.
A standard microplate spectrophotometer set to 450 nm (reference: 540–570 nm) is required. An automated plate washer reduces wash-step variability. Calibrate and warm up your reader before measuring. Blank using the zero-standard or buffer-only well before constructing the standard curve.
Store the unopened kit at 2–8 °C until the expiry date. Shelf life: Traditional: 12 months | Ready-to-use: 16 months from date of manufacture. Reconstituted standard is stable at 4 °C for ≤1 week; prepare fresh for critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
