Gentamicin ELISA Kit

What it means: Assay Type describes how the kit generates its signal and whether it reports concentrations. For this kit the assay type is Quantitative, so each well is read against the standard curve. Standards and samples are run on the same plate and compared through the standard curve.

Why it matters:

• Each plate needs its own standard series.
• Knowing the format helps when choosing curve-fitting settings in the analysis software.

How to interpret here: For this Quantitative assay, run the full standard series on every plate and calculate sample values from that plate's own curve; a four-parameter logistic fit is a common choice.

Tip: Run standards and samples in duplicate and include a blank on every plate.

What it means: Sample Type lists the sample matrices recommended for this kit: Serum, Plasma, Cell Culture Supernatant. Each matrix can affect background and recovery differently, so the sample type should match the matrix used in the study.

Why it matters:

• Lipids, hemoglobin and anticoagulants in a sample can change background and recovery.
• Following the recommended dilution helps keep readings within the standard curve.
• Collection, storage and freeze-thaw history can affect results, so handle all samples in a study the same way.

How to interpret here: For Serum, Plasma & more, follow the sample handling and dilution guidance on the datasheet; check dilution linearity and spike recovery before testing many samples in a new matrix.

Tip: For Serum, Plasma & more, compare results only between samples of the same matrix and avoid repeated freeze-thaw cycles.

What it means: Precision describes how repeatable the results are within one plate (intra-assay CV) and between plates or runs (inter-assay CV). For this kit the precision is Intra-CV <15% / Inter-CV <15%. Lower CV values mean more consistent replicate readings.

Why it matters:

• Good precision makes small differences between samples easier to trust.
• Intra-assay CV reflects pipetting and plate uniformity; inter-assay CV reflects day-to-day and lot-to-lot variation.
• Precision values describe the kit under the stated conditions; handling in each laboratory also affects the CV.

How to interpret here: With precision of Intra-CV <15% / Inter-CV <15%, run samples in duplicate and repeat any pair whose CV is much higher than these values.

Tip: Use the same pipettes, timing and washer settings for all plates in one study.

What it means: Recovery shows how much of a known amount of analyte added to a sample is measured back. For this kit the recovery is 80-120%. Values close to 100% indicate that the sample matrix interferes little with the assay.

Why it matters:

• Recovery outside the expected range points to matrix effects in the sample.
• Checking recovery is most useful when a new sample type or dilution is introduced.
• Recovery is matrix-dependent, so values can differ between serum, plasma and other fluids.

How to interpret here: With a recovery of 80-120%, spike a known amount of standard into a representative sample and check that the measured value falls in a similar range before testing many samples.

Tip: Run spike recovery at the same dilution that will be used for the study samples.