GES-1 cell

SKU:BHC11101510
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Overview
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GES-1 cell is a Epithelial cell cell line (Unspecified). It is commonly used as an in vitro model for 2 research. Growth characteristics: Adherent. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Growth Properties Adherent
Tissue Fetal Stomach
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Catalog no. Size
305428 1 cryovial
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This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 305428
Species Human
GES-1 is a human gastric epithelial cell line commonly used in research focused on the gastric mucosa, particularly in studies exploring gastric diseases, inflammation, and cytotoxic responses. These cells are derived from normal gastric tissue and provide an in vitro model for investigating the effects of environmental toxins, drugs, and pathogens on gastric epithelial cells. One significant area of research utilizing GES-1 involves studying the cytotoxic effects of environmental pollutants, such as nanoplastics, on human gastric cells. For example, polystyrene nanoplastics (PS-NPs) have been shown to enter GES-1 cells via endocytosis, inducing cellular stress responses such as autophagy, apoptosis, and decreased cell proliferation. These particles were found to accumulate in vesicles, autophagosomes, and lysosomes, indicating their internalization and cytotoxic potential within gastric epithelial cells. Additionally, studies have shown that inhibiting pathways like the RhoA/F-actin signaling pathway reduces the internalization of these nanoplastics, which helps in understanding the molecular mechanisms governing cellular uptake and response to foreign particles. GES-1 cells are also used to investigate the protective effects of various compounds against gastric injuries. For instance, the traditional medicinal plant Fallopia denticuta has demonstrated protective effects on GES-1 cells against ethanol-induced damage. The study showed that extracts of this plant enhanced the proliferation of GES-1 cells and reduced oxidative stress and inflammation, which are key contributors to gastric ulcer development. This makes GES-1 an important tool for exploring both cytotoxic mechanisms and potential protective treatments in gastric health research.

SKU:BHC11101510

  • Tumorigenic: No (tested in nude mice)
  • Viruses: Transformant: Simian virus 40 (SV40)
  • cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
  • supplements: Supplement the medium with 10% FBS
  • dissociationReagent: Accutase
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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