| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Storage |
Affinity purified recombinant enhanced green fluorescent protein (GFP) is a versatile tool that can be effectively used as a control to determine transfection efficiency, for microinjection into tissues and cells as well as for expression studies. GFP Purified Protein can be used as a standard for SDS gels, for Western Blots, and for fluorometry. This protein has a 6X Histidine Tag at its N-terminus and was purified using immobilized metal affinity chromatography (IMAC).
| Specification | Value |
|---|---|
| Enzyme Type | Reporter Protein |
| Format | Enzyme Only |
| Storage Conditions | The lyophilized protein is stable for at least one year from date of receipt at -20°C. Upon reconstitution, this protein can be stored in working aliquots at 2°-8°C for one week. Avoid repeated freeze/thaw cycles. |
Each lot is functionally tested to verify activity prior to release. A Certificate of Analysis (CoA) documenting lot-specific quality control data is available on request.
Store at −20°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles; aliquoting for long-term storage is recommended. This product is stable for ≥2 years from the date of shipment under proper storage conditions.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
References
- McPartlon TJ; Osborne CT; Wang K et al.. An Ultrapotent, Ultraeconomical, Antifreeze Polypeptide. Advanced materials (Deerfield Beach, Fla.). 2026;38:e20504. DOI
