| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GK5 recombinant protein (Position: D37-K402) was used as the immunogen for the GK5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GK5 Antibody / Glycerol kinase 5 is a anti-GK5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: GK5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Glycerol kinase 5 is distinct from the canonical cytosolic glycerol kinase (GK1) in its subcellular localization and tissue specificity. It is highly expressed in testis and skin, where it may regulate lipid composition during spermatogenesis and barrier formation. The GK5 antibody allows detection of this enzyme in mitochondria-rich tissues, helping define its contribution to energy production and lipid biosynthesis. By phosphorylating glycerol, GK5 supports the glycerol phosphate shuttle and links carbohydrate metabolism with lipid anabolism.
Structurally, Glycerol kinase 5 shares conserved ATP-binding and catalytic residues with other glycerol kinases but contains mitochondrial targeting sequences. It associates with mitochondrial membranes, where it modulates local glycerol metabolism. The GK5 antibody supports subcellular localization studies that examine mitochondrial lipid synthesis and oxidative phosphorylation coupling. In skin and reproductive tissues, GK5 may play roles in lipid barrier maintenance and sperm energy metabolism.
Altered expression of Glycerol kinase 5 has been observed in metabolic disorders and certain cancers. Its involvement in lipid signaling suggests that GK5 activity could affect membrane dynamics and cellular energy balance. The GK5 antibody facilitates quantification of expression changes under metabolic stress, nutrient deprivation, or differentiation. Functional studies indicate that GK5 may participate in adaptive metabolic responses that sustain energy production under shifting substrate conditions.
The GK5 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing punctate mitochondrial staining consistent with its predicted localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
