| Field | Specification |
|---|---|
| Alternative names | N4-Spermine cholesteryl carbamate |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C38H70N4O2 |
| SMILES | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
GL67, also known as N4-Spermine cholesteryl carbamate, is a cationic lipid that serves as a non-viral vector for siRNA delivery and gene transfer. It enhances the encapsulation efficiency of siRNA in liposomal formulations, promotes cellular uptake of siRNA across cancer cell membranes, and mediates transduction of plasmid DNA into cells, and can be used in drug-delivery research[1][2]. It has the molecular formula C38H70N4O2 (MW 614.99).
Physical & Chemical Properties
| CAS Number | 179075-30-0 |
|---|---|
| Molecular Formula | C38H70N4O2 |
| Molecular Weight | 614.99 g/mol |
| SMILES | C[C@@]12[C@]3([H])[C@](CC=C1C[C@H](CC2)OC(N(CCCN)CCCCNCCCN)=O)([H])[C@@]4([H])[C@](CC3)([C@@](CC4)([H])[C@H](C)CCCC(C)C)C |
| Signaling Pathway | Metabolic Enzyme/Protease |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with your institution's chemical hygiene plan.
In Vitro
As the cationic lipid-to-siRNA molar ratio rises from 1:1 to 3:1, GL67 lipid complexes show larger particle sizes and positively shifted zeta potentials; the 100% GL67 formulation at 3:1 produces a high positive zeta potential of 47 mV. GL67 raises siRNA encapsulation efficiency across all tested ratios, and complete encapsulation is reached with less GL67 at higher lipid-siRNA ratios[1]. At a 3:1 cationic lipid/siRNA molar ratio, the GL67 pentahydrochloride-based lipid complex shows favorable metabolic and membrane integrity compatibility with A549 cells and increases cellular uptake of Cy3-siRNA in A549 cells[1]. In differentiated, polarized primary human and canine tracheobronchial epithelial cells grown at the air-liquid interface, GL67 (GL-67) mediated markedly lower gene transfection efficiency, together with suppressed cell division and elevated transepithelial electrical resistance. Transfection capacity fell as cell confluence rose in plate-grown unpolarized human bronchial epithelial cells, and also fell in primary human bronchial epithelial cells that were growth-arrested and poorly proliferative[2].
In Vivo
In the nasal epithelium of cystic fibrosis mice, GL67:pCF1-CFTR complexes (0.6 mM GL67 combined with 3.6 mM pCF1-CFTR; nasal perfusion) achieve only marginal, partial correction of the Cl− transport defect[2]. In the lungs of healthy BALB/c mice, GL67:pDNA complexes (0.6 mM GL67 combined with 3.6 mM pCF1-CAT; intranasal bolus (lungs); nasal perfusion) drive >1000-fold higher gene expression than naked pDNA, while gene expression in the nasal epithelium is enhanced only minimally and without statistical significance[2].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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