| Field | Specification |
|---|---|
| Mfr No | |
| Product Type |
Goat Anti-Mouse IgG Plates are specifically designed to bind the Fc region of mouse immunoglobulin subclasses (IgG1, IgG2a, IgG2b, IgG3) with minimal cross-reactivity to human, bovine, and rabbit serum proteins. These plates are particularly useful for binding assays where antibody quantities are limited, or where antibodies may denature and lose activity when directly adsorbed to polystyrene surfaces.
The Goat Anti-Mouse IgG Coated Plates prevent antibody denaturation caused by direct adsorption on polystyrene, ensuring antibodies remain active. Unlike Protein A or G plates, they provide selective binding specifically to target mouse IgG, offering higher specificity. Additionally, these plates deliver enhanced antibody-binding capacity, especially when using diluted mouse antibody solutions, outperforming direct adsorption to polystyrene.
Key Features
- Enhanced Binding Capacity: Offers superior antibody-binding capacity compared to direct adsorption on polystyrene, particularly when using diluted mouse antibody solutions.
- Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
- Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
- Wells design: flat bottom.
- Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
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Colors: Available in clear, white, and black. Customizable upper rim colors upon request. Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays. Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
- Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
- Alphanumeric Coding: For easy and accurate well identification during experiments.
- Recommended working volume: of 75 to 200 μl
- Packaging: Each coated plate is packed in a single barrier bag with desiccant
- Unit: Contains 5 plates
- Minimum order: 10 plates.
- Ready to use
Key Benefits
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Plates Design: The mould design provides optical quality, important to reduce the background signal. The rim protects the external face of the bottom from scratches
- The mould design provides optical quality, important to reduce the background signal.
- The rim protects the external face of the bottom from scratches
- Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
- Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
- Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
- Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.
Applications
- Sandwich ELISA: Provides a stable solid support for capturing mouse IgG and detecting non-mouse antibodies.
- Competitive ELISA: Suitable for quantitative detection of target analytes.
- IgG Isotyping: Facilitates accurate classification of IgG subclasses.
- Hybridoma Screening & Selection: Supports efficient screening for monoclonal antibody production.
- Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
- Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
- Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.
- Others
- Chen YJ et al. (2018). Development of a highly sensitive enzyme-linked immunosorbent assay for the detection of Zika virus IgG antibodies using a protein G capture platform. J Virol Methods. 263:55-62. PMID: 30552393.
- Hamilton RG et al. (1988). Monoclonal antibody-based immunoenzymetric assays for quantification of IgG subclasses. J Immunol Methods. 107(2):235-244. PMID: 3069872.