Goat Anti-Rabbit IgG (H&L); AF555 conjugated

SKU:BHA10575527
Overview
Click light‑blue chips for details
IgG-specific polyclonal antibody raised in Goat. Alexa Fluor 555-conjugated. Validated for ELISA, Immunohistochemistry, Immunofluorescence in Rabbit samples. Affinity-purified.
Conjugate Alexa Fluor 555
Immunogen Native Protein
Applications 4 validated
Dilution ELISA:1:5000-1:10000, IH:1:100-1:1000, I
Options selector
Catalog no. Size
CSB-PA273173-100UL 100 uL
CSB-PA273173-500UL 500 uL
Available Options
Size
Field Specification
Mfr No CSB-PA273173
Antigen
  • IgG purified from Rabbit serum
Clonality
  • Polyclonal
Conjugate
  • AF555
Form Liquid
Host Goat
Immunogen IgG purified from Rabbit serum
Isotype
  • IgG
Product Type
  • Primary Antibody
Reactivity
  • Rabbit
Source This product is a polyclonal antibody purified from goat antiserum.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target IgG

Product Overview

Primary Antibody

This antibody targets IgG and was raised against a recombinant protein immunogen.

Validated Applications

Application Recommended Dilution
ELISA ELISA:1:5000-1:10000, IH:1:100-1:1000, IF:1:100-1:1000, FC:1:100-1:4000
Immunohistochemistry (IHC) ELISA:1:5000-1:10000, IH:1:100-1:1000, IF:1:100-1:1000, FC:1:100-1:4000
Immunofluorescence (IF) ELISA:1:5000-1:10000, IH:1:100-1:1000, IF:1:100-1:1000, FC:1:100-1:4000
Flow Cytometry (FC) ELISA:1:5000-1:10000, IH:1:100-1:1000, IF:1:100-1:1000, FC:1:100-1:4000
✔ Validated by CUSABIO Technology

Antibody Specifications

Target IgG
Host Goat
Clonality Polyclonal
Isotype IgG
Conjugate Alexa Fluor 555
Reactivity Rabbit
Purification Affinity Purification
Antigen / Immunogen IgG purified from Rabbit serum
Production This product is a polyclonal antibody purified from goat antiserum.
Form Liquid
Storage Buffer PBS, 0.2% sodium azide, 50% glycerol, 0.1% BSA, pH 7.2
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle with standard laboratory precautions. Dispose per local regulations.
TargetIgG
HostGoat
ClonalityPolyclonal
IsotypeIgG
ConjugateAlexa Fluor 555
ReactivityRabbit
PurificationAffinity Purification
Antigen/ImmunogenIgG purified from Rabbit serum
ProductionThis product is a polyclonal antibody purified from goat antiserum.
FormLiquid
Storage BufferPBS, 0.2% sodium azide, 50% glycerol, 0.1% BSA, pH 7.2
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Dilutions are starting recommendations. Titration is advised for each system.

ApplicationRecommended Dilution
ELISAELISA:1:5000-1:10000, IH:1:100-1:1000, IF:1:100-1:1000, FC:1:100-1:4000
Immunohistochemistry (IHC)ELISA:1:5000-1:10000, IH:1:100-1:1000, IF:1:100-1:1000, FC:1:100-1:4000
Immunofluorescence (IF)ELISA:1:5000-1:10000, IH:1:100-1:1000, IF:1:100-1:1000, FC:1:100-1:4000
Flow Cytometry (FC)ELISA:1:5000-1:10000, IH:1:100-1:1000, IF:1:100-1:1000, FC:1:100-1:4000

Immunogen: IgG purified from Rabbit serum

Antigen Species: Rabbit

What species does this IgG antibody react with?
This antibody has been validated for reactivity against Rabbit. Cross-reactivity is based on the immunogen sequence and may vary by sample type.
What applications has this antibody been validated for?
Validated applications include: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC). Each application was confirmed with appropriate controls. Contact technical support for applications not listed.
What is the recommended dilution?
ELISA:1:5000-1:10000, IH:1:100-1:1000, IF:1:100-1:1000, FC:1:100-1:4000. Optimal concentrations depend on sample type, detection system, and antigen abundance. Titration with positive and negative controls is recommended.
How was this antibody produced?
This polyclonal IgG antibody was raised in Goat and purified by affinity chromatography using Protein G. It provides high specificity and minimal cross-reactivity in standard immunoassay applications.
What are the storage requirements?
Store at −20°C for long-term. Avoid repeated freeze-thaw cycles. Short-term working aliquots may be stored at 4°C. This product is supplied in a stabilized buffer containing BSA and sodium azide.
Is this antibody directly conjugated and does it require secondary detection?
Yes, this antibody is Alexa Fluor 555-conjugated and can be used directly without a secondary antibody. It is ready to use in assays requiring Alexa Fluor 555 detection, such as flow cytometry or direct ELISA applications. Ensure your detection instrument is calibrated for Alexa Fluor 555 signal.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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