| Field | Specification |
|---|---|
| Mfr No | |
| Antigen | |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Rabbit IgG(H+L) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from goat antiserum. |
| Storage | |
| Target |
Product Overview
Primary AntibodyThis antibody targets IgG and was raised against a recombinant protein immunogen.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| ELISA | WB:1:5000-20000, IHC:1:200-2000, IF:1:200-2000, IP:1:200-1:2000, FCM:1:200-2000, ELISA:1:5000-20000 |
| Western Blot (WB) | WB:1:5000-20000, IHC:1:200-2000, IF:1:200-2000, IP:1:200-1:2000, FCM:1:200-2000, ELISA:1:5000-20000 |
| Immunohistochemistry (IHC) | WB:1:5000-20000, IHC:1:200-2000, IF:1:200-2000, IP:1:200-1:2000, FCM:1:200-2000, ELISA:1:5000-20000 |
| Immunofluorescence (IF) | WB:1:5000-20000, IHC:1:200-2000, IF:1:200-2000, IP:1:200-1:2000, FCM:1:200-2000, ELISA:1:5000-20000 |
| Immunoprecipitation (IP) | WB:1:5000-20000, IHC:1:200-2000, IF:1:200-2000, IP:1:200-1:2000, FCM:1:200-2000, ELISA:1:5000-20000 |
| Flow Cytometry (FC) | WB:1:5000-20000, IHC:1:200-2000, IF:1:200-2000, IP:1:200-1:2000, FCM:1:200-2000, ELISA:1:5000-20000 |
Antibody Specifications
| Target | IgG |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Biotin |
| Reactivity | Rabbit |
| Purification | Affinity Purification |
| Antigen / Immunogen | Rabbit IgG(H+L) |
| Production | This product is a polyclonal antibody purified from goat antiserum. |
| Form | Liquid |
| Storage Buffer | PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide, pH 7.4. |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Safety & Handling
⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle with standard laboratory precautions. Dispose per local regulations.
—
| Target | IgG |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Biotin |
| Reactivity | Rabbit |
| Purification | Affinity Purification |
| Antigen/Immunogen | Rabbit IgG(H+L) |
| Production | This product is a polyclonal antibody purified from goat antiserum. |
| Form | Liquid |
| Storage Buffer | PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide, pH 7.4. |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Dilutions are starting recommendations. Titration is advised for each system.
| Application | Recommended Dilution |
|---|---|
| ELISA | WB:1:5000-20000, IHC:1:200-2000, IF:1:200-2000, IP:1:200-1:2000, FCM:1:200-2000, ELISA:1:5000-20000 |
| Western Blot (WB) | WB:1:5000-20000, IHC:1:200-2000, IF:1:200-2000, IP:1:200-1:2000, FCM:1:200-2000, ELISA:1:5000-20000 |
| Immunohistochemistry (IHC) | WB:1:5000-20000, IHC:1:200-2000, IF:1:200-2000, IP:1:200-1:2000, FCM:1:200-2000, ELISA:1:5000-20000 |
| Immunofluorescence (IF) | WB:1:5000-20000, IHC:1:200-2000, IF:1:200-2000, IP:1:200-1:2000, FCM:1:200-2000, ELISA:1:5000-20000 |
| Immunoprecipitation (IP) | WB:1:5000-20000, IHC:1:200-2000, IF:1:200-2000, IP:1:200-1:2000, FCM:1:200-2000, ELISA:1:5000-20000 |
| Flow Cytometry (FC) | WB:1:5000-20000, IHC:1:200-2000, IF:1:200-2000, IP:1:200-1:2000, FCM:1:200-2000, ELISA:1:5000-20000 |
Immunogen: Rabbit IgG(H+L)
Antigen Species: Rabbit
What species does this IgG antibody react with?
This antibody has been validated for reactivity against Rabbit. Cross-reactivity is based on the immunogen sequence and may vary by sample type.
What applications has this antibody been validated for?
Validated applications include: ELISA, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC). Each application was confirmed with appropriate controls. Contact technical support for applications not listed.
What is the recommended dilution?
WB:1:5000-20000, IHC:1:200-2000, IF:1:200-2000, IP:1:200-1:2000, FCM:1:200-2000, ELISA:1:5000-20000. Optimal concentrations depend on sample type, detection system, and antigen abundance. Titration with positive and negative controls is recommended.
How was this antibody produced?
This polyclonal IgG antibody was raised in Goat and purified by affinity chromatography using Protein G. It provides high specificity and minimal cross-reactivity in standard immunoassay applications.
What are the storage requirements?
Store at −20°C for long-term. Avoid repeated freeze-thaw cycles. Short-term working aliquots may be stored at 4°C. This product is supplied in a stabilized buffer containing BSA and sodium azide.
Is this antibody directly conjugated and does it require secondary detection?
Yes, this antibody is Biotin-conjugated and can be used directly without a secondary antibody. It is ready to use in assays requiring Biotin detection, such as flow cytometry or direct ELISA applications. Ensure your detection instrument is calibrated for Biotin signal.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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