| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Biological Activity | |
| Enzyme Type | |
| Expression System | |
| Form | Liquid |
| Formulation | |
| Product Type | |
| Purity | |
| Source | E.coli. |
| Species | |
| Storage | |
| Target |
Product Description
Glutamic-Pyruvate Transaminase 2 Human Recombinant, Active
Overview
Alanine aminotransferase 2 (GPT2), catalyzes the reversible transamination among alanine and 2-oxoglutarate to create pyruvate and glutamate. GPT2 expressed mainly in muscle, fat and kidney and participates in the intermediary metabolism of glucose and amino acids. Multiple transcript variants encoding various isoforms have been found for GPT2.
Biological Activity
Specific activity is > 100units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C.
Specifications
| Purity | Greater than 90% as determined by SDS-PAGE. |
|---|---|
| Formulation | The GPT2 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH7.5), 30% glycerol, 2mM DTT, 0.2M NaCl. |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Expression System | E.coli. |