| Field | Specification |
|---|---|
| Target | |
| Alternative names | Growth factor receptor-bound protein 10|GRB10 adapter protein|Insulin receptor-binding protein Grb-IR|GRB10|GRBIR|KIAA0207 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | growth factor receptor-bound protein 10 |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin(INSR) and insulin-like growth factor(IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes GRB10. It is validated for ELISA, Western Blot, Immunofluorescence, Immunohistochemistry.
Validated Applications
| Applications | ELISA, Western Blot, Immunofluorescence, Immunohistochemistry |
|---|---|
| WB | 1:500-1:2000 |
| IHC | 1:20-1:200 |
| IF | 1:20-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
growth factor receptor-bound protein 10
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | growth factor receptor-bound protein 10 |
| Purification | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 65 kDa |
| UniProt # | Q13322 |
| Gene ID | 2887 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Alternative Names | Growth factor receptor-bound protein 10|GRB10 adapter protein|Insulin receptor-binding protein Grb-IR|GRB10|GRBIR|KIAA0207 |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for GRB10 research from Fine Test, or contact us for alternative formats.
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