| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
The glucocorticoid response element (GRE) is a DNA sequence bound by the glucocorticoid receptor (GR), a ligand-activated nuclear receptor. Upon binding glucocorticoid hormones such as cortisol, GR translocates to the nucleus, dimerizes, and binds GRE sequences to activate or repress target gene transcription. Glucocorticoid signaling controls metabolism, immune and inflammatory responses, stress adaptation, and development, and is the basis for the widely used anti-inflammatory and immunosuppressive actions of synthetic glucocorticoids. A GRE-driven reporter provides a direct readout of this nuclear hormone receptor pathway, supporting research into endocrine signaling, anti-inflammatory drug action, and steroid receptor pharmacology.
Product Description & Applications
The GRE Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of glucocorticoid receptor signaling in human or mouse cells. The glucocorticoid response element drives reporter expression in response to glucocorticoid-activated GR, so signal intensity reflects nuclear hormone pathway activity. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is applied to study glucocorticoid signaling, GR pharmacology, anti-inflammatory drug responses, and steroid hormone biology. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.
About This Product
This reporter lentivirus places a Firefly Luc, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Nuclear hormone receptor pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
