| Field | Specification |
|---|---|
| Target | |
| Alternative names | GRP78, GRP-78, Grp78, GRP 78, HSPA5, HSPA 5, Heat shock 70 kDa protein 5, Heat Shock 70kDa Protein 5, BiP, BIP, Binding immunoglobulin protein, Immunoglobulin heavy chain-binding protein, Immunoglobulin Heavy Chain Binding Protein, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Endoplasmic reticulum lumenal, Ca2+ binding protein grp78, Glucose Regulated Protein 78kDa, 78 kDa glucose-regulated protein, 78 kDa glucose regulated protein, HSCe70, MIF2, mBiP, Sez7, AL022860, AU019543, D2Wsu141e, D2Wsu17e, FLJ26106, GRP78_HUMAN |
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| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Full length human GRP78 (Bip) his tagged at the N terminus |
| Purification | |
| Cellular localization | |
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GRP78 (also known as BiP) is a 78-kDa glucose-regulated protein and a master regulator of the unfolded protein response (UPR). As a key ER chaperone, it ensures proper protein folding, assembly, and the clearance of misfolded proteins, especially under stress conditions.
Its expression is controlled by an internal ribosome entry site (IRES), which becomes highly active during cellular stress, such as heat shock or oxidative damage. GRP78 is essential for maintaining neuronal homeostasis and preventing apoptosis, making it a critical player in neurodegenerative disease contexts.
In neuroscience, GRP78 has demonstrated neuroprotective effects against glutamate toxicity and oxidative stress. Its levels are notably reduced in the brains of Alzheimer’s disease patients, highlighting its potential as a biomarker and therapeutic target.
Additionally, GRP78 supports embryonic development and pluripotent cell survival, further underscoring its importance in neural cell viability. However, its overexpression under extreme stress may contribute to drug resistance in neuro-oncology, adding complexity to its therapeutic modulation.
0.5 µg/ml of SPC-180 was sufficient for detection of Grp78 in 10 µg of rat tissue lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cite this product varies by variant:
- SPC-180D — Size: 100 ug: GRP78 (Bip) Antibody (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SPC-180D, RRID: AB_10804549)
- SPC-180D-A390 — Size: 100 ug: GRP78 (Bip) Antibody: ATTO 390 (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SPC-180D-A390, RRID: AB_2703908)
- SPC-180D-A488 — Size: 100 ug: GRP78 (Bip) Antibody: ATTO 488 (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SPC-180D-A488, RRID: AB_2703909)
- SPC-180D-A594 — Size: 100 ug: GRP78 (Bip) Antibody: ATTO 594 (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SPC-180D-A594, RRID: AB_2703911)
- SPC-180D-APC — Size: 100 ug: GRP78 (Bip) Antibody: APC (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SPC-180D-APC, RRID: AB_2703917)
- SPC-180D-BI — Size: 100 ug: GRP78 (Bip) Antibody: Biotin (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SPC-180D-BI, RRID: AB_2703918)
- SPC-180D-FITC — Size: 100 ug: GRP78 (Bip) Antibody: FITC (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SPC-180D-FITC, RRID: AB_2703919)
- SPC-180D-HRP — Size: 100 ug: GRP78 (Bip) Antibody: HRP (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SPC-180D-HRP, RRID: AB_2703920)
- SPC-180D-PCP — Size: 100 ug: GRP78 (Bip) Antibody: PerCP (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SPC-180D-PCP, RRID: AB_2703922)
- SPC-180D-RPE — Size: 100 ug: GRP78 (Bip) Antibody: RPE (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SPC-180D-RPE, RRID: AB_2703923)
- SPC-180S — Size: 12 ug: GRP78 (Bip) Antibody (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SPC-180S, RRID: AB_10804549)
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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