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| Main SKU |
This kit is for measuring the activity of H. influenzae DNA primase. The enzyme-included version supplies the H. influenzae primase-helicase complex together with the DNA template, NTP mix, assay buffer and fluorescence dye.
Kit vs Kit Plus
The only difference is the enzyme: HGA100K supplies the assay reagents without enzyme, while HGA100KE adds 45 µl of 100 x H. influenzae primase-helicase complex. Both cover 100 assays in a 96-well plate or 200 assays in a 384-well plate.
| Kit (enzyme not included), 100 assays (HGA100K) | 100 assays (96-well) or 200 assays (384-well) |
|---|---|
| Kit Plus (enzyme included), 100 assays (HGA100KE) | 100 assays (96-well) or 200 assays (384-well) |
Kit Components
| Component | Kit (enzyme not included), 100 assays (HGA100K) | Kit Plus (enzyme included), 100 assays (HGA100KE) |
|---|---|---|
| 10 x Assay buffer | 600 µl | 600 µl |
| 100 x DNA template | 45 µl | 45 µl |
| 100 x NTP mix | 45 µl | 45 µl |
| 10 x Fluorescence dye | 850 µl | 850 µl |
| 100 x H. influenzae primase-helicase complex | Not included | 45 µl |
Specifications
| Assay Target | H. influenzae DNA primase activity |
|---|---|
| Target Type | DNA Primase |
| Organism | H. influenzae |
| Assay Type | Enzyme Activity Assay |
| Detection Method | Fluorescence |
| Plate Format | 96-well or 384-well plate |
| Size | 100 assays (96-well) or 200 assays (384-well) |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol
A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this H. influenzae DNA primase activity assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.