| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Zeocin, Puromycin, Blasticidin, N/A |
| Shipping | |
| Species |
Background
AHR (aryl hydrocarbon receptor) is a ligand-activated, basic helix-loop-helix-PAS transcription factor. In its inactive state it resides in the cytosol bound to chaperone proteins. Upon binding ligands, including environmental xenobiotics such as 2,3,7,8-tetrachlorodibenzo-p-dioxin as well as dietary and microbial metabolites, AHR translocates to the nucleus and dimerizes with the AHR nuclear translocator to activate genes encoding xenobiotic-metabolizing enzymes. Beyond detoxification, AHR regulates immune cell differentiation, intestinal barrier function, cell proliferation, and inflammatory responses. Dysregulated AHR signaling is implicated in toxic responses, chronic inflammation, autoimmune conditions, and cancer, making the receptor an important target in toxicology, immunology, and cancer research.
Product Description & Applications
The h/m AHR shRNA Lentivirus delivers a validated short hairpin RNA targeting human and mouse AHR for stable, RNA interference-based gene silencing. The 19-20 bp shRNA is expressed from a U6 Pol III promoter within a third-generation, self-inactivating lentiviral backbone, with a constitutively expressed fluorescent reporter (GFP, GFP/Luc, RFP, RFP/Luc) and an antibiotic selection marker (Zeocin, Puromycin, or Blasticidin) for tracking and stable line generation. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cells. Knockdown is confirmed to meet or exceed 70% using a fluorescence-based assay. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation. Sets supply lentivirus from two independent shRNAs plus a scrambled-shRNA control, supporting loss-of-function studies of AHR in cancer and cell biology.
About This Product
This validated shRNA lentivirus targeting AHR delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP/Luc, RFP, RFP/Luc) and antibiotic selection marker (Zeocin, Puromycin, Blasticidin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.
Knockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
