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| Selection Marker | Blasticidin, N/A, Puromycin |
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Background
HOXA9 (homeobox A9) is a homeodomain transcription factor belonging to the HOX family of master regulators of body patterning and tissue identity. In the hematopoietic system HOXA9 is highly expressed in immature progenitor cells, where it supports self-renewal and proliferation, and is normally downregulated as cells differentiate. Sustained or aberrant HOXA9 expression blocks differentiation and promotes the expansion of leukemic cells. HOXA9 overexpression is a recurrent feature of acute myeloid leukemia, frequently driven by chromosomal rearrangements involving MLL or by upstream regulators such as NUP98 fusions, and it is associated with poor prognosis. HOXA9 is therefore widely studied as a key oncogenic transcription factor in leukemia research.
Product Description & Applications
The h/m HOXA9 shRNA Lentivirus delivers a validated short hairpin RNA targeting human and mouse HOXA9 for stable, RNA interference-based gene silencing. The 19-20 bp shRNA is expressed from a U6 Pol III promoter within a third-generation, self-inactivating lentiviral backbone, with a constitutively expressed fluorescent reporter (GFP, GFP/Luc, RFP, RFP/Luc) and an antibiotic selection marker (Blasticidin or Puromycin) for tracking and stable line generation. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cells. Knockdown is confirmed to meet or exceed 70% using a fluorescence-based assay. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation. Sets supply lentivirus from two independent shRNAs plus a scrambled-shRNA control, supporting loss-of-function studies of HOXA9 in leukemia and cell biology.
About This Product
This validated shRNA lentivirus targeting HOXA9 (NCBI Accession: NM_152739.4) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, RFP, GFP/Luc, RFP/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.
Knockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
