| Field | Specification |
|---|---|
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
Neuropilin 1 (NRP1) is a single-pass transmembrane glycoprotein that functions as a co-receptor for multiple ligand families, including class 3 semaphorins and members of the VEGF family. By partnering with plexins and VEGF receptors, NRP1 modulates axon guidance, vascular development, and angiogenesis. NRP1 is also expressed on immune cells, where it influences regulatory T cell function and dendritic cell activity. In cancer, NRP1 promotes tumor angiogenesis, cell migration, and invasion, and contributes to an immunosuppressive microenvironment. These roles make NRP1 a focus of research in vascular biology, neurodevelopment, immunology, and oncology.
Product Description & Applications
The h/m NRP1 shRNA Lentivirus delivers validated short hairpin RNA targeting human and mouse NRP1 from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping enables broad tropism across primary, suspension, and cryopreserved cells, and each shRNA is validated for at least 70% NRP1 knockdown by a fluorescence-based method.
High-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option supplies a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of angiogenesis, axon guidance, immune regulation, and tumor progression.
About This Product
This validated shRNA lentivirus targeting NRP1 (NCBI Accession: NM_003873.7) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP/Luc, RFP, RFP/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.
Knockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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