| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
SERCA2 (sarcoplasmic/endoplasmic reticulum Ca2+-ATPase 2, encoded by ATP2A2) is a P-type ion pump that transports calcium from the cytosol into the sarcoplasmic and endoplasmic reticulum, maintaining intracellular calcium homeostasis. In cardiac and skeletal muscle, SERCA2 is essential for relaxation by clearing cytosolic calcium after each contraction, and it also supports endoplasmic reticulum calcium stores in non-muscle cells. Reduced SERCA2 activity is associated with impaired cardiac contractility and heart failure, while loss-of-function mutations cause the skin disorder Darier disease. SERCA2 is therefore an important target in cardiovascular, calcium-signaling, and cell biology research.
Product Description & Applications
The h/m SERCA2 shRNA Lentivirus provides high-titer lentiviral particles for stable knockdown of human and mouse SERCA2. The shRNA is delivered from a third-generation, self-inactivating backbone with expression from a U6 Pol III promoter, alongside a constitutively expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and an antibiotic selection marker. VSV-G pseudotyping enables broad tropism, including primary, suspension, and cryopreserved cells. The shRNA is validated to achieve at least 70% knockdown using a fluorescence-based assay. The set comprises lentivirus produced from a mix of two independent validated shRNAs plus a matched scrambled-shRNA control. It is used for loss-of-function studies of SERCA2 in calcium homeostasis, muscle physiology, and cardiovascular research.
About This Product
This validated shRNA lentivirus targeting SERCA2 delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, RFP, GFP/Luc, RFP/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.
Knockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
