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| Selection Marker | Blasticidin, N/A, Puromycin |
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Background
TSP1 (thrombospondin 1, encoded by THBS1) is a large secreted matricellular glycoprotein that mediates cell-matrix and cell-cell interactions. TSP1 is best known as a potent endogenous inhibitor of angiogenesis, acting in part through the receptor CD36 to suppress endothelial cell proliferation and migration. It also activates latent TGF-β, regulates platelet aggregation, and influences inflammation, wound healing, and tissue remodeling. By restraining new blood vessel formation and modulating the extracellular matrix, TSP1 shapes tumor growth and the microenvironment, making it a frequently studied target in cancer, angiogenesis, and cell biology research.
Product Description & Applications
The h/m TSP1 shRNA Lentivirus provides high-titer lentiviral particles for stable knockdown of human and mouse TSP1. The shRNA is delivered from a third-generation, self-inactivating backbone with expression from a U6 Pol III promoter, alongside a constitutively expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and an antibiotic selection marker. VSV-G pseudotyping enables broad tropism, including primary, suspension, and cryopreserved cells. The shRNA is validated to achieve at least 70% knockdown using a fluorescence-based assay. The set comprises lentivirus produced from a mix of two independent validated shRNAs plus a matched scrambled-shRNA control. It is used for loss-of-function studies of TSP1 in angiogenesis, the extracellular matrix, and cancer research.
About This Product
This validated shRNA lentivirus targeting TSP1 delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, RFP, GFP/Luc, RFP/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.
Knockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
