| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
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| Species |
Background
USP20 (ubiquitin specific peptidase 20) is a deubiquitinating enzyme that removes ubiquitin chains from target proteins, thereby protecting them from proteasomal degradation and regulating their stability and activity. As part of the ubiquitin-proteasome system, USP20 influences diverse processes, including hypoxia signaling through stabilization of HIF1α, β2-adrenergic receptor recycling, DNA damage responses, and inflammatory and metabolic signaling. By counteracting ubiquitin ligases, deubiquitinases such as USP20 fine-tune the abundance of key regulatory proteins. Altered USP20 activity has been associated with cancer progression and metabolic dysfunction, making it an informative target for studies of protein turnover and signal transduction.
Product Description & Applications
The h/m USP20 shRNA Lentivirus delivers high-titer shRNA lentiviral particles that specifically silence human and mouse USP20. The shRNA is expressed from a third-generation, self-inactivating lentiviral backbone and has been validated to achieve at least 70% knockdown using a rapid fluorescence-based assay rather than qPCR. Co-expressed fluorescent reporters (GFP or RFP, with optional luciferase) and antibiotic selection markers (puromycin or blasticidin) allow tracking of transduced cells and rapid establishment of stable knockdown lines. The shRNA lentivirus set provides particles from a mix of two independent validated shRNAs plus a matched scrambled-shRNA control. Ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells, for loss-of-function studies of USP20 and deubiquitinase biology.
About This Product
This validated shRNA lentivirus targeting USP20 (NCBI Accession: NM_006676.8) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP/Luc, RFP, RFP/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.
Knockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
