| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
SIGLEC5 (sialic acid-binding immunoglobulin-like lectin 5) is a member of the SIGLEC family of cell-surface receptors that recognize sialylated glycans and largely transmit inhibitory signals. Expressed mainly on myeloid cells such as neutrophils and monocytes, SIGLEC5 contains ITIM motifs in its cytoplasmic tail that recruit phosphatases to dampen immune cell activation. By engaging sialic acid ligands on host and pathogen surfaces, SIGLECs help discriminate self from non-self and contribute to immune tolerance. Altered sialoglycan-SIGLEC interactions have been implicated in inflammation, infection, and tumor immune evasion, making SIGLEC5 a target of interest in glycoimmunology and cancer immunology research.
Product Description & Applications
The h SIGLEC5 shRNA Lentivirus delivers a validated short hairpin RNA targeting human SIGLEC5 to achieve stable gene knockdown. The shRNA is expressed from a self-inactivating lentiviral backbone alongside a fluorescent reporter (GFP or RFP, with optional luciferase) and an antibiotic selection marker, enabling generation of stable knockdown cell lines. Knockdown of at least 70% has been confirmed using a fluorescence-based assay that provides a more rapid and reliable readout than qPCR.
The shRNA lentivirus set provides particles produced from a mix of two independent validated shRNAs plus a matched scrambled-shRNA control. Applications include loss-of-function studies of SIGLEC5 in immune signaling and cancer biology. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.
About This Product
This validated shRNA lentivirus targeting SIGLEC5 delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP/Luc, RFP, RFP/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.
Knockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
