H19 Dye for DNA Relaxation and Supercoiling Assays

SKU:BHT16300181
Overview
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Fluorescent dye that distinguishes relaxed from supercoiled DNA for high-throughput DNA supercoiling and relaxation assays of topoisomerases and inhibitor screens. 170 µl of 1500 x dye for 1,000 assays in 96-well plates.
Assays per Pack 1,000 assays
Concentration 1500 x
Shipping Frozen
Options selector
Catalog no. Size
DSA1000D 1,000 assays (170 µl, 1500 x)
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 1,000 assays (170 µl, 1500 x) – DSA1000D
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Please contact us to confirm storage conditions.
  • Shipping: Shipped frozen.
  • Upon receipt: unpack promptly.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Applications
  • Enzyme Activity
Detection wavelength Ex 485 nm / Em 535 nm
Concentration 1500 x
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.
Catalog no. (Mfr.) DSA1000D
Main SKU BHT16300181
DNA Topology Dyes

Product Overview

H19 dye is a fluorescent dye designed for high-throughput DNA supercoiling and relaxation assays. DNA gyrases convert relaxed circular DNA into supercoiled DNA, and DNA topoisomerase I converts supercoiled DNA into relaxed DNA.

In the presence of magnesium, relaxed DNA suppresses H19 fluorescence much more than supercoiled DNA, so the signal rises when relaxed DNA is supercoiled and falls when supercoiled DNA is relaxed. The difference is used to measure DNA topology changes by topoisomerases and to screen topoisomerase inhibitors in 96-well plates; the assay can be adapted to 384-well plates.

Product Description

1,000 assays (170 µl, 1500 x) – DSA1000D 170 µl of 1500 x H19 dye for 1,000 DNA relaxation or supercoiling assays

Specifications

Concentration 1500 x
Volume 170 µl
Assays 1,000 (96-well plate format)
Excitation / Emission 485 nm / 535 nm
Use with 10 x H19 Dilution Buffer (BHT16300178)
Catalog # DSA1000D
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.

The ProFoldin protocol “H19 Dye for DNA Relaxation and Supercoiling Assays” is linked under Documents.

Notes

  • Magnesium is required for the assay; avoid EDTA in the reaction.

Safety & Handling

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.
Q.What is supplied?
A.170 µl of 1500 x H19 dye, enough for 1,000 DNA relaxation or supercoiling assays in a 96-well plate format (catalog # DSA1000D).
Q.How does the assay work?
A.With magnesium present, relaxed DNA suppresses H19 fluorescence more than supercoiled DNA, so supercoiling raises the signal and relaxation lowers it.
Q.Which plate formats can I use?
A.The assay is designed for 96-well plates and can be adapted to 384-well plates.
Q.Which wavelengths should I use?
A.Excitation at 485 nm and emission at 535 nm.
Q.Is a protocol available?
A.Yes. The ProFoldin protocol “H19 Dye for DNA Relaxation and Supercoiling Assays” is linked under Documents.
Q.How is it shipped and stored?
A.Shipped frozen. Please contact us to confirm storage conditions.

Can’t find the exact format you need? We can help you source or customize this H19 Dye for DNA Relaxation and Supercoiling Assays — including larger pack sizes or bulk orders. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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