| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
Histone H3.1, encoded by H3C1, is a canonical core histone deposited into chromatin in a replication-coupled manner during S phase. Together with H4, H2A, and H2B it forms the nucleosome, the fundamental unit of chromatin that packages genomic DNA. The amino-terminal tail of H3 carries post-translational modifications that regulate gene expression, DNA repair, and genome stability. Recurrent point mutations in histone H3 genes, sometimes called oncohistones, alter chromatin states and have been identified as drivers in certain cancers, where they reprogram the epigenetic landscape and block normal differentiation. Comparing wild-type and mutant H3.1 provides a powerful approach for studying chromatin biology, epigenetic regulation, and oncohistone-driven tumorigenesis.
Product Description & Applications
The H3.1 (WT & Mutant) ORF cDNA Lentivirus enables stable expression of wild-type or mutant human histone H3.1 (H3C1) in mammalian cells. Lentiviral particles are packaged with an expression vector in which an EF1a or CMV promoter drives the H3.1 open reading frame fused to a C-terminal V5 epitope tag, with a fluorescent reporter (GFP or RFP) and a selection marker (Puromycin or Blasticidin) separated from the ORF by self-cleaving peptide sequences for independent translation. In some configurations a PGK promoter drives the reporter and selection cassette. The third-generation, VSV-G pseudotyped particles transduce a wide range of cells, including primary and cryopreserved cells, supporting reliable genome integration and stable line generation. ORF accuracy is confirmed by sequencing and expression validated by transfection. The product supports gain-of-function studies of chromatin biology and oncohistone function.
About This Product
This ORF cDNA lentivirus enables stable overexpression of H3C1 (NCBI Accession: NM_003529) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (EF1a). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.
Ultra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
