HAL-01 cell

SKU:BHC11101220
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Overview
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HAL-01 cell is a cell line (Female). It is commonly used as an in vitro model for 1 research. Growth characteristics: Suspension, Lymphoblast. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Morphology Lymphoblast
Growth Properties Suspension
Tissue B Cell Precursor Leukemia
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Catalog no. Size
305140 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 305140
Species Human
The HAL-01 cell line is derived from the peripheral blood of a female adolescent with a diagnosis of acute lymphoblastic leukemia (ALL), specifically the L2 subtype. This cell line is particularly notable for containing the t(17;19)(q22;p13) chromosomal translocation, which results in the TCF3-HLF (E2A-HLF) fusion gene. This genetic feature is critical in the study of leukemia as it influences the behavior of leukemia cells, including aspects of their growth, differentiation, and response to therapies. The presence of the TCF3-HLF fusion gene in the HAL-01 cell line makes it an invaluable resource for oncological research, particularly for studies focused on the mechanisms of leukemogenesis and the development of targeted therapies for leukemia. The fusion protein encoded by this gene is involved in the regulation of gene transcription and has been associated with poor prognosis in patients, underscoring the importance of this cell line in therapeutic development and prognostic research in acute lymphoblastic leukemia.

SKU:BHC11101220

  • cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
  • supplements: Supplement the medium with 10% FBS
  • doublingTime: 48 hours
  • subculturing: Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 105 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation.
  • fluidRenewal: 2 to 3 times per week
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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