| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human HAUS8 recombinant protein (Position: D86-A322) was used as the immunogen for the HAUS8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HAUS8 Antibody / HAUS augmin-like complex subunit 8 is a anti-HAUS8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: HAUS8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
HAUS8 is encoded by the HAUS8 gene located on human chromosome 19q13.32. The protein is approximately 42 kilodaltons and localizes to spindle microtubules during mitosis. HAUS8 interacts with other augmin subunits (HAUS1-7) and the gamma-tubulin ring complex, facilitating branching microtubule nucleation within the spindle. This activity is crucial for maintaining spindle symmetry and attachment of kinetochores to microtubules during metaphase.
The HAUS8 antibody detects a 42 kilodalton band by western blot and shows distinct spindle staining by immunofluorescence. Depletion of HAUS8 disrupts spindle microtubule amplification, resulting in defective chromosome alignment and prolonged mitosis. Cells lacking HAUS8 exhibit reduced kinetochore tension and increased aneuploidy, underscoring its importance in maintaining genomic stability.
HAUS8 also functions in interphase cells, where it contributes to microtubule organization and centrosome positioning. Dysregulation of HAUS8 has been observed in cancers with chromosomal instability, suggesting that aberrant spindle nucleation may drive tumor progression. Because augmin complex activity is essential for spindle integrity, HAUS8 serves as a critical factor linking microtubule dynamics with mitotic checkpoint control.
Through its role in spindle formation and genome maintenance, HAUS8 represents a key target for studies of cell division, cancer biology, and microtubule regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
