| Field | Specification |
|---|---|
| Mfr No | |
| Species |
The HEK293A cell line, a derivative of the human embryonic kidney 293 (HEK293) cells, represents a specialized tool in virological and gene therapy research, particularly in the production, amplification, and titration of replication-incompetent adenoviruses. These cells exhibit a flat morphology, which significantly aids in the microscopic examination and titration processes, making it simpler to count and assess viral particles.
A pivotal feature of the HEK293A cell line is the stable integration of the adenovirus E1 gene into its genome. This integration is critical as it provides the necessary transcriptional machinery for the expression of E1 proteins, specifically E1a and E1b. The presence of these proteins is essential for the replication of adenoviral vectors in the cell. The E1a protein primarily functions to activate transcription of the adenovirus genome, while E1b proteins are involved in viral replication and cell cycle disruption.
The utility of HEK293A cells extends beyond merely supporting viral replication. These cells facilitate the efficient production of high titer, high-quality viral preparations essential for both basic research and therapeutic applications. The cell line's robust replication capacity and ease of handling enable researchers to screen and develop adenoviral constructs with unprecedented precision and efficiency.
In summary, the HEK293A cell line is an indispensable resource in the field of virology and gene therapy. Its ability to stably express E1 proteins and support adenoviral replication makes it a valuable tool for researchers looking to produce and manipulate adenoviral vectors. The cell line's characteristics allow for the efficient generation of viral vectors, crucial for advancing research and potential therapeutic interventions.
—
- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
10%
OFF
15%OFF
20%
OFF
$50
OFF
FREE
SAMPLE
