| Field | Specification |
|---|---|
| Target | |
| Alternative names | Haemoglobin |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Rabbit Hemoglobin (Native Protein) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from guinea pig antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This is a polyclonal anti-Hemoglobin antibody raised in Guinea pig, conjugated with HRP, with confirmed utility in ELISA. Designed to detect Hemoglobin in Rabbit, supporting researchers in others contexts.
Key elements and design rationale
- Immunogen: Rabbit Hemoglobin (Native Protein) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.
- Host (Guinea pig): Requires anti-guinea pig-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.
- Polyclonal: Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.
- Isotype (IgG): Secondary antibodies and isotype controls must match IgG from Guinea pig. Relevant for multiplexing and Protein A/G compatibility.
- Purification (Protein G purification): Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.
- HRP conjugate: Direct chromogenic (TMB/OPD) or chemiluminescent (ECL) detection. Quench endogenous peroxidase in tissue applications. No secondary required.
Biological background
Hemoglobin (also known as Haemoglobin) is a protein studied in Oryctolagus cuniculus (Rabbit) systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of Hemoglobin biology relevant to others.
Common research applications
- ELISA: Sandwich or indirect ELISA quantifies soluble/secreted target in biological fluids or cell culture supernatants.
Notes for experimental interpretation
- Isotype controls: Include IgG from Guinea pig at equivalent concentration to assess non-specific background.
- Cross-reactivity/isoforms: Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.
- Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.
- Species reactivity: Confirmed for Rabbit; extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from guinea pig antiserum.
Hemoglobin is a protein target in Rabbit biology. This polyclonal antibody raised in Guinea pig detects Hemoglobin and is validated for ELISA applications.
This antibody is reported reactive against Rabbit. Immunogen derived from Oryctolagus cuniculus (Rabbit). Cross-reactivity with unlisted species should not be assumed — contact us or consult the datasheet for homology data.
Supplier reports performance in ELISA. Dilution optimization, blocking, and appropriate controls are required per format. Contact BioHippo for recommended working concentrations.
HRP enables direct chromogenic (TMB/OPD) or chemiluminescent (ECL) detection — no secondary needed. Block endogenous peroxidase in tissue applications before applying this antibody.
(1) Isotype control — IgG from Guinea pig at matching concentration; (2) Positive control — lysate/cell line expressing Hemoglobin; (3) Negative control — siRNA KD or KO sample to confirm specificity; (4) Dilution linearity — verify proportional signal decrease for quantitative applications.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
