| Field | Specification |
|---|---|
| Product Type |
Heparin Catcher HC1 Plates are specifically designed for in vitro measurement of unfractionated heparin in low-protein fluids using ELISA assays. The specialized surface of these plates immobilizes and measures heparin levels within a range of 0.01 to 2.0 U/ml. Heparin, a well-studied glycosaminoglycan known for its anticoagulant properties, is commonly used in both its native unfractionated form (UFH) and as low molecular weight (LMW) heparin. These heparin ELISA assays are competitive, with the colorimetric signal inversely proportional to the heparin concentration in the sample, ensuring accurate detection.
Heparin Catcher HC1 Coated 96 Well Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.
Key Features
- Heparin Detection:It can detect concentrations ranging from 0.01 to 2.0 U/ml, making it ideal for accurate and sensitive assays.
- Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
- Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
- Wells design: flat bottom.
- Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
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Colors: Available in clear, white, and black. Customizable upper rim colors upon request. Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays. Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
- Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
- Alphanumeric Coding: For easy and accurate well identification during experiments.
- Recommended working volume: of 50 to 100 μl
- Packaging: Each coated plate is packed in a single barrier bag with desiccant
- Unit: Contains 5 plates
- Minimum order: 10 plates.
- Ready to use
Key Benefits
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Plates Design: The mould design provides optical quality, important to reduce the background signal. The rim protects the external face of the bottom from scratches
- The mould design provides optical quality, important to reduce the background signal.
- The rim protects the external face of the bottom from scratches
- Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
- Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
- Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
- Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.
Applications
- ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
- Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
- Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
- Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.
- Wang S et al. (2024). Low-molecular-weight heparin ameliorates intestinal barrier dysfunction in a murine model. Int Immunopharmacol. 138:112557. PMID: 38970715.
- Huang L et al. (2020). Preventive application of low molecular weight heparin ameliorates peripheral neuropathy via targeting inflammation. Exp Neurol. 330:113340. PMID: 32269677.
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