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HEY Cells, derived from a human ovarian cancer xenograft, are a valuable resource for cancer researchers seeking to advance their understanding of papillary cystadenocarcinoma, a moderately differentiated form of ovarian cancer. The parental cell line, HEY, was initially obtained from a peritoneal sample of a Caucasian patient diagnosed with this specific type of cancer. These epithelial-like cells closely resemble human cells, making them an excellent model for studying ovarian cancer. HEY, Cells exhibit a rapid doubling time of approximately 30 hours, allowing for efficient and time-effective experimentation. Researchers can utilize these cells to investigate various aspects of cancer biology, such as tumour formation, metastasis, and drug response.
HEY, Cells are particularly well-suited for applications involving 3D cell culture, a technique that more closely mimics the physiological environment of tumours. Their ability to grow in semisolid culture and as xenografts in immunologically deprived CBA/CJ mice highlights their adaptability and potential for in vivo studies. By incorporating HEY Cells into cancer research, scientists can uncover crucial insights into the development and progression of papillary cystadenocarcinoma. These cells are invaluable for exploring novel therapeutic strategies, identifying potential drug targets, and evaluating treatment efficacy.
In summary, HEY Cells provide researchers with a robust and reliable resource for investigating ovarian cancer. With their origins in a patient sample and their epithelial-like morphology, these cells faithfully replicate key characteristics of papillary cystadenocarcinoma. Their applications in 3D cell culture and cancer research make them essential in advancing our understanding of this challenging disease.
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Tumorigenic: Yes
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- doublingTime: 20 to 30 hours
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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