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Hifair™ Advanced Multiplex One Step RT-qPCR Probe Mix (UDG Plus) is a reagent kit designed for multiplex quantitative PCR (qPCR) using RNA as the template. In this workflow, reverse transcription and qPCR are performed in a single reaction tube, simplifying experimental procedures and reducing the risk of contamination.
This kit employs a thermostable Reverse Transcriptase for efficient first-strand cDNA synthesis and HotStart Taq DNA Polymerase for quantitative amplification. The kit includes an optimized MP Buffer and Enzyme Mix. The buffer already contains Mg²⁺ and dNTPs, along with components that effectively suppress non-specific PCR amplification and enhance the efficiency of multiplex qPCR reactions—enabling robust multiplex amplification while maintaining primer amplification efficiency. Additionally, the dUTP/UDG system is incorporated to effectively prevent aerosol-mediated contamination.
Features
- All-in-One Master Mix: Buffers and enzymes precombined in a single tube for one-step RT-qPCR setup — pipette and run.
- Exceptional Stability: Maintains performance after accelerated aging at 37°C for 7 days and ≥20 freeze–thaw cycles, enabling reliable storage and transport.
- dUTP/UDG anti-pollution system: Incorporates dUTP/UDG enzyme to prevent amplicon carryover contamination for cleaner results.
Applications
- RT-qPCR Detection
- Diagnostic pathogen detection (respiratory tract, intestinal tract, etc.)
- animal pathogen detection (non-swine fever, etc.)
- food testing (microorganisms, etc.).
Hifair™ Advanced Multiplex One Step RT-qPCR Probe Mix (UDG Plus) is a reagent kit designed for multiplex quantitative PCR (qPCR) using RNA as the template. In this workflow, reverse transcription and qPCR are performed in a single reaction tube, simplifying experimental procedures and reducing the risk of contamination. This kit employs a thermostable Reverse Transcriptase for efficient first-strand cDNA synthesis and HotStart Taq DNA Polymerase for quantitative amplification. The kit includes an optimized MP Buffer and Enzyme Mix. The buffer already contains Mg²⁺ and dNTPs, along with components that effectively suppress non-specific PCR amplification and enhance the efficiency of multiplex qPCR reactions—enabling robust multiplex amplification while maintaining primer amplification efficiency. Additionally, the dUTP/UDG system is incorporated to effectively prevent aerosol-mediated contamination.
Key Features
All-in-One Master Mix: Buffers and enzymes precombined in a single tube for one-step RT-qPCR setup — pipette and run. Exceptional Stability: Maintains performance after accelerated aging at 37°C for 7 days and ≥20 freeze–thaw cycles, enabling reliable storage and transport. dUTP/UDG anti-pollution system: Incorporates dUTP/UDG enzyme to prevent amplicon carryover contamination for cleaner results.
Kit Components
Name 16921ES50 16921ES60 16921ES80 16921ES92 5×RT-qPCR Master Mix 250 μL 500 μL 5 mL 50 mL Storage This product should be stored at -25~-15℃ for 1 year. Application RT-qPCR Detection; Diagnostic pathogen detection (respiratory tract, intestinal tract, etc.); animal pathogen detection (non-swine fever, etc.); food testing (microorganisms, etc.).
Storage & Shipping
Storage: -20°C | Shipping: Dry Ice
