| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Storage |
Description
The HieffTM Blood RNA Kit is suitable for the extraction of RNA from samples such as blood, plasma, serum, and lymph fluid. The silica-based material used in the centrifugal adsorption column is a unique new material developed by our company. It, combined with our unique lysis buffer formulation, allows for the maximum recovery of high-purity RNA. The extracted RNA has high purity, stable and reliable quality, and can be used for various downstream applications, such as RT-PCR, RT-qPCR, in vitro transcription, molecular cloning, etc.
Features
Fast and simple: simple operation, single sample extraction is completed in 30 minutes.
Safe and non-toxic: no isopropanol extraction is required.
Application Blood samples: suitable for RNA extraction from blood, plasma, serum, lymph and other samples.
Components
|
part |
Components No. |
Name |
19241ES50 |
|
Part I |
19241-A |
Lysis Buffer / LB Buffer B3 |
50 mL |
|
Part II |
19241-B |
HieffTM RNA Column B3 |
50 columns |
|
19241-C |
2 mL Collection Tube B3 |
50 tubes |
|
|
19241-D |
Deproteinized Solution / PL Buffer B3 |
25 mL |
|
|
19241-E |
Wash Buffer* |
12 mL |
|
|
19241-F |
RNase-free H2O |
5 mL |
Shipping and Storage
This product should be transported at room temperature. Upon receipt:
Part I: Store at 4°C, protected from light.
Part II: Store at room temperature.
The product is valid for one year.
Application
RNA extraction from blood, plasma, serum, or lymph samples.
Figures
Whole Blood RNA Extraction
Table 1. Concentration and purity QC results of the extracted RNA
|
Sample No. |
Concentration (ng/μL) |
OD260/280 |
OD260/230 |
Extraction reagents |
|
1 |
18.24 |
1.99 |
0.92 |
Yeasen-19241 |
|
2 |
18.14 |
2.01 |
1.17 |
|
|
3 |
25.23 |
1.76 |
0.60 |
|
|
4 |
11.57 |
1.39 |
0.14 |
|
|
5 |
13.54 |
1.47 |
0.11 |
Supplier M* |
|
6 |
14.51 |
1.49 |
0.11 |
|
Six 250 μL fresh blood samples were extracted using 19241 and M brand kits, with RNA eluted in 50 μL RNase-free water. 1 μL of each eluate was used to measure RNA concentration.
Result: 19241 showed higher RNA yield.
Figure 1. Blood RNA Integrity QC Results
Six 250 μL fresh blood samples were extracted using 19241 and M brand kits, with RNA eluted in 50 μL RNase-free water. 5 μL of each eluate was analyzed for RNA integrity and band intensity.
Result: RNA extracted with 19241 showed better integrity and clearer bands.
Documents:
Safety Data Sheet
Manuals
19241_Manual_Ver.EN20250605.pdf
Citations & References:
[1] Yang Y, Liu S, He C, et al. LncRNA LYPLAL1-AS1 rejuvenates human adipose-derived mesenchymal stem cell senescence via transcriptional MIRLET7B inactivation. Cell Biosci. 2022;12(1):45. Published 2022 Apr 21. doi:10.1186/s13578-022-00782-x(IF:7.133)
[2] Yan S, Sun M, Gao L, et al. Identification of Key LncRNAs and Pathways in Prediabetes and Type 2 Diabetes Mellitus for Hypertriglyceridemia Patients Based on Weighted Gene Co-Expression Network Analysis. Front Endocrinol (Lausanne). 2022;12:800123. Published 2022 Jan 24. doi:10.3389/fendo.2021.800123(IF:5.555)
[3] Sun M, Yan S, Zhao D, et al. Identified lncRNAs functional modules and genes in prediabetes with hypertriglyceridemia by weighted gene co-expression network analysis. Nutr Metab (Lond). 2022;19(1):33. Published 2022 May 2. doi:10.1186/s12986-022-00665-5(IF:4.169)
[4] Qi Y, Yuan M, Yi Q, et al. A panel of two miRNAs correlated to systolic blood pressure is a good diagnostic indicator for stroke. Biosci Rep. 2021;41(1):BSR20203458. doi:10.1042/BSR20203458(IF:3.840)
