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Hieff Canace™ Veritas Long PCR Master Mix (No Dye) is a ready-to-use 2× premix designed for long-fragment amplification with 83-fold higher fidelity than standard Taq DNA polymerase and 9-fold higher than standard Pfu (NGS-validated). Extension rate of 10 sec/kb supports amplification up to 40 kb from genomic templates. Amplification products have blunt ends. Supports templates with 20–80% GC content and enables direct amplification from whole blood (EDTA, citrate, or heparin) without prior nucleic acid extraction.
Features
- 83× higher fidelity than Taq; 9× higher than standard Pfu — NGS-validated
- Long-fragment amplification: up to 40 kb from genomic DNA
- Blunt-end products: directly compatible with blunt-end cloning and Gibson Assembly
- Broad GC range: 20–80% GC content templates
- Direct amplification from whole blood without prior nucleic acid extraction
- High sensitivity: validated across 1 pg to 1 µg template input
Performance Summary
- Genomic DNA: up to 40 kb; extension 5 sec/kb (≤10 kb) or 10 sec/kb (>10 kb)
- cDNA: efficient amplification of 1–14 kb fragments
- Sensitivity: serial dilution from 1 pg to 1 µg
For research use only.
This High-Fidelity DNA Polymerase delivers 83-fold higher fidelity than standard Taq DNA polymerase and 9-fold higher than standard Pfu DNA polymerase. Fidelity was evaluated by next-generation sequencing (NGS), which provides a direct and highly sensitive error rate measurement across thousands of base positions simultaneously.
This mix can amplify genomic DNA fragments up to 40 kb. Extension rate guidance: use 5 sec/kb for fragments up to 10 kb, and 10 sec/kb for fragments greater than 10 kb. cDNA fragments from 1 kb to 14 kb can also be amplified efficiently.
Yes. This formulation enables direct amplification from crude whole blood samples without prior nucleic acid extraction. Compatible blood preservation anticoagulants include EDTA, citrate, and heparin. Residual inhibitors in crude blood are tolerated by the optimized buffer system.
Amplification products have blunt ends. This makes the products directly compatible with blunt-end cloning, Gibson Assembly, and other ligation-independent cloning methods without any additional polishing or A-tailing steps.
This mix is validated for templates with GC content ranging from 20% to 80%, making it suitable for amplification of both GC-rich and AT-rich templates including challenging genomic regions and repetitive sequences.
Yeasen Biotechnology supports custom formulation and scale-up services for reagent mixes and master mixes. Contact BioHippo to discuss project requirements and initiate an inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen reagent mixes are available in GMP grade for IVD assay development. Manufactured in an ISO 13485-certified UCF.ME™ facility with FDA Drug Master File (DMF) support.
- GMP release testing and CoA documentation
- Scalable from milliliter to liter-scale quantities
- Consistent lot-to-lot performance specifications
▶ Glycerol-Free & Lyophilization-Ready Formats
Glycerol-free and lyophilization-ready formats are available for reagents intended for cartridge-based point-of-care devices, liquid handling automation, or ambient-temperature distribution.
- Glycerol-free liquid formulation
- Lyophilization-compatible format with cryoprotectant
- Custom reaction buffer optimization
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting team provides end-to-end support for molecular diagnostic product development using Yeasen reagent mixes as a foundation.
- Master mix selection and performance matching for target assay
- Primer/probe design and assay buffer optimization
- Sensitivity, specificity, and LOD validation
- Instrument compatibility assessment
▶ Custom Formulation
Custom formulation services are available for researchers requiring modifications to standard reagent mix compositions, including ROX level adjustment, dye substitution, stabilizer optimization, and buffer modification.
- ROX concentration customization (No / Low / High ROX)
- Reference dye and indicator dye options
- Buffer composition and pH optimization
- Scale-up to commercial quantities on confirmation
ⓘ Customization services fulfilled by Yeasen Biotechnology. Contact BioHippo for project scoping and pricing.
