| Field | Specification |
|---|---|
| Product Type | |
| Shipping | |
| Storage |
Description
This product is suitable for multiple RT-PCR experiments，and it is suitable for co-detection of DNA&RNA pathogenic microorganisms.This kit is based on super multiplex RT-PCR, combined with hot start modification technology and matched with the optimal buffer system, which can realize the re-amplification of Panel with panel multiplicity of 1-2,000. This product is compatible with reverse transcription system, and the GC content of amplicon can cover the range of 25-75%. It has the characteristics of high uniformity, high specificity and high sensitivity. This reagent is suitable for multiple amplification of fragments with different lengths, such as 100-500 bp, and can also be used for the capture of amplicon more than thousand.
Features
Simplify operation: Packing the pre-mixed solution , and only need to add templates and primers in the experiment.RT-PCR is combined into one step.
Widely compatibility: compatible with microbial research, pathogen detection, genetic disease detection and agricultural detection etc.
Excellent amplification performance: high detection rate, excellent specificity and good uniformity.
Strict control of background bacteria: clean workshop, professional personnel and strict quality control.
Applications
tNGS；Microbial detection；Multiplex PCR；RT-PCR
Specifications
|
Cat.No. |
12950ES24/ 12950ES48 / 12950ES96 |
|
Size |
24 T / 48 T / 96 T |
Components
|
Components No. |
Name |
12950ES24 |
12950ES48 |
12950ES96 |
12950ES98 |
|
12950-A |
RT-PCR buffer |
168 μL |
336 μL |
672 μL |
7 mL |
|
12950-B |
RT-PCR Enzyme mix |
12 μL |
24 μL |
48 μL |
500 μL |
|
12950-C |
4X PCR mix |
180 μL |
360 μL |
720 μL |
7.5 mL |
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year.
Figures
Figure1. Detection of microorganisms DNA &RNA
Libraries were prepared using Cat#12950protocol ,To evaluate the detection of DNA and RNA species with100 copies/T RNA Virus Standard and 10 pg the ZymoBIOMICS Microbial Community DNA Standard (Zymo Research #D6306). The results show that the performance of 12950 is better than other suppliers.
Documents:
Safety Data Sheet
Manuals
12950_Manual_Ver.EN20250123.pdf
Citations & References:
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