Hieff NGS™ 1st Strand Synthesis Kit

SKU:BHT20800027
Research Validated
Overview
Click light‑blue chips for details
This product can be used to synthesize one-strand cDNA of full-length. After the synthesis of the second strand cDNA, the product can be directly to construct sequencing library.
Kit Category Process / Workflow Kits
Grade RUO
Storage -20°C
Shipping Dry Ice
Options selector
Catalog no. Size
12946ES08 24 T
12946ES24 48 T
12946ES96 96 T
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size (3) - 24 T, 48 T, 96 T
  • Lead time: options listed in “Availability Content”; otherwise, there will be a column of “lead time”, other statuses may take longer.
  • Storage: -20°C
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No 12946ES
Product Type
  • First-Strand cDNA Synthesis Kit
Shipping Dry Ice
Storage -20°C

Description

This product can be used to synthesize one-strand cDNA of full-length. After the synthesis of the second strand cDNA, the product can be directly to construct sequencing library.

Features

Rapid reverse transcription: Complete the cDNA synthesis process within 30 min.

Widely compatibility: After reverse transcription, multiple amplification can be performed directly without purification.

Strict control of background bacteria:  clean workshop, professional personnel and strict quality control.

Applications

tNGS;Microbial detection;Reverse Transcription;cDNA Synthesis

Specifications

Cat.No.

12950ES24/ 12950ES48 / 12950ES96

Size

24 T / 48 T / 96 T

Components

Components No.

Name

12946ES08

12946ES24

12946ES96

12946-A

Random Primer

16 μL

48 μL

192 μL

12946-B

10×1st Reaction Buffer

16 μL

48 μL

192 μL

12946-C

1st Strand Enzyme Mix

16 μL

48 μL

192 μL

 

Shipping and Storage

This product should be stored at -25~-15℃ for one year.

Figures

Figure1. Excellent reverse transcription efficiency. Different RNA virus targets were simultaneously reverse transcribed with 12946, and the obtained cDNA products were quantitatively analyzed by qPCR. The results showed that the performance of 12946 was better than that of other suppliers. 

Figure1. Excellent reverse transcription efficiency. Different RNA virus targets were simultaneously reverse transcribed with 12946, and the obtained cDNA products were quantitatively analyzed by qPCR. The results showed that the performance of 12946 was better than that of other suppliers.

Documents:

Safety Data Sheet

12946_MSDS_HB250331_EN.PDF

Manuals

12946_Manual_Ver.EN20250331.pdf

Citations & References:

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today