| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Storage |
Description
The Hieff DNA Library Prep Kit for ONT is a DNA library preparation kit developed for the third-generation Nanopore sequencing platform. This kit has undergone targeted modifications and optimizations to its enzymes and buffer system, significantly improving the ligation efficiency of long fragments. Additionally, after end repair and A-tailing, no purification is required, allowing direct Barcode ligation, making the experimental workflow more convenient.
Applications
- DNA Library Construction
- Third-Generation Library Construction
- TGS Library Construction
- Nanopore Sequencing
- ONT Sequencing
Product Advantages
Ease of Operation: End repair and A-tailing completed in one step, no purification required, allowing direct ligation with Barcode adapters
Wide Compatibility: Compatible with various sample types including animals, plants, and microorganisms; supports both gDNA and amplicon samples
Excellent Data Performance: High library yield and superior sequencing data quality
Components
|
No. |
Name |
13301ES08 |
13301ES24 |
13301ES96 |
|
8 T |
24 T |
96 T |
||
|
13301-A |
Endprep Buffer |
56 μL |
168 μL |
672 μL |
|
13301-B |
Endprep Enzyme |
24 μL |
72 μL |
288 μL |
|
13301-C |
Rapid Ligase Master Mix |
280 μL |
840 μL |
4×840 μL |
|
13301-D |
Rapid Ligation Reaction Buffer（5×） |
160 μL |
480 μL |
2×960 μL |
|
13301-E |
Rapid T4 DNA Ligase |
40 μL |
120 μL |
480 μL |
|
13301-F |
Elution Buffer |
160 μL |
480 μL |
2×960 μL |
Storage Conditions
Store at -25°C to -15°C, with a shelf life of 1 year.
Precautions
1. The washing reagents used for purification after Native Barcode ligation and Native Adapter purification are different. Please ensure the correct reagents are used for each purification step.
2. To ensure successful library construction, it is recommended to verify the fragment length and integrity of the input DNA (validation methods include capillary electrophoresis or other equivalent methods). DNA quality (integrity, fragmentation), purity (e.g., chemical contamination, RNA residuals), and excessive or insufficient usage may affect library yield.
3. Please prepare all necessary materials in advance according to the provided guidelines to avoid issues that could prevent proper execution of the experiment, leading to sample or reagent waste.
4. When preparing libraries for multiple samples, please record the Native Barcode number corresponding to each sample.
Workflow
Case Study
|
Template |
Input DNA |
Recovery |
Data Volume |
Reads mean length |
Reads max length |
Reads N50 length |
|
Human gDNA (4-20 K) |
1μg |
96% |
15 G |
9136.1715 |
113518 |
12633 |
|
1.7 K |
500 ng |
65% |
0.33G |
1791 |
23493 |
1771 |
|
3 K |
500 ng |
75% |
0.31G |
2557 |
26165 |
3005 |
|
1-16 K |
1 μg |
34.5% |
2.89G |
4189 |
41457 |
5893 |
|
Plasmid 1 |
300 ng |
＞60% |
0.96 G |
2413 |
23637 |
2658 |
|
Plasmid 2 |
300 ng |
＞60% |
0.83 G |
2129 |
35855 |
2808 |
Documents:
Safety Data Sheet
Manuals
