| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Storage |
Description
Hieff NGSTM EvoMax RNA Library Prep Kit (dNTP) is designed for total RNA sequencing library preparation and is compatible with both Illumina and MGI platforms. Available in two formats—standard tube and pre-loaded plate—the plate format eliminates manual preparation and sub-packaging, enabling direct integration with automated library construction for greater convenience. The kit includes reagents for RNA fragmentation, reverse transcription, ds-cDNA synthesis, and library amplification. It can be seamlessly combined with mRNA purification or rRNA depletion kits. All components undergo rigorous quality control and functional validation to ensure stable, reproducible library preparation results.
Features
- Premixed Reagents: Enzymes and buffers are pre-mixed to reduce reagent components and simplify preparation.
- Safe and light-stable: The non-specific reagent requires no additional light protection, ensuring safe handling and consistent, reliable results.
- Preloaded Plate Format—Ready to Use: Designed for seamless integration with automation instruments, the preloaded plates enable easy “tear-and-use” operation for convenient automated workflows.
Components
|
Components No. |
Name |
12341ES08 |
12341ES24 |
12341ES96 |
12341ES97(Tube Automation 96T) |
12341ES98(Plate Automation 96T) |
|
12341-A |
Frag/Prime Buffer |
150 μL |
450 μL |
2× |
2× |
8× |
|
12341-B |
1st Reaction Module 2.0 |
64 μL |
192 μL |
768 μL |
960 μL |
8×120 μL |
|
12341-C |
2nd Reaction Module(dNTP) |
280 μL |
840 μL |
3× |
3× |
8× |
|
12341-D |
Ligation Reaction Module |
280 μL |
840 μL |
3× |
3× |
8×480 μL |
|
12341-E |
2×Super CanaceTM II High-Fidelity Mix |
200 μL |
600 μL |
2× |
2× |
8× |
|
* |
Primer Mix* |
/ |
/ |
/ |
/ |
/ |
[Note]: * The notation indicates that the component is not included in the kit. Primer mix is required if the complete adapters are used, otherwise it isn’t. This kit is compatible with Illumina and MGI platforms, but need additional primer mix (Cat # 13334 Primer Mix for MGI and Cat # 13335 Primer Mix for Illumina) for Illumina or MGI platform if the complete adapters are used.
** See the following diagram for the layout of plate reagent group.
Storage
This product should be stored at -25~-15℃ for one year..
Application
RNA library Preparation; Transcriptome profiling and gene expression analysis; mRNA sequencing for coding RNA studies; lncRNA (long non-coding RNA) research; Small RNA sequencing (if applicable); Differential gene expression analysis; RNA biomarker discovery; RNA variant and fusion detection.
Figures
1.Simplified Product Format
Figure 1. Simplied components of EvoMax RNA Library Prep Kit.
2.Fast RNA Library preparation
Figure 2. Premixed RNA Library Prep: More Efficient and Time-Saving
3.Automation-friendly
Figure 3. Highly uniform mRNA and lncRNA libraries prepared with 96-channel automation.
4.Strong Stability
Figure 4. Real-time stability: After 18 months of storage, performance remains comparable to the reference kit.
Documents:
Safety Data Sheet
Manuals
