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This kit is a ready-to-use 2× premixed solution containing High-Fidelity DNA Polymerase, dNTP s, and the buffer system carefully optimized for amplification of fragments within 20 Kb, with an extension rate of 10 sec/kb. Its fidelity is 83 times that of Taq DNA polymerase and 9 times that of Pfu DNA polymerase. It is suitable for complex template amplification, and the amplified product has a blunt end.
This product is fast and simple, highly sensitive, specific and stable. It can amplify fragments with a GC content of 30% to 70%. It can amplify whole blood samples that have not been extracted with nucleic acids. It is suitable for blood preserved with EDTA, citrate or heparin, as well as dried blood spot cards, which is faster and more flexible. In addition, this product also contains a specific protective agent, so the premix can maintain stable activity after repeated freezing and thawing. All reagent components provided in this product have undergone strict quality inspection to ensure the excellent performance and batch-to-batch stability of the product to the highest extent. This reagent is suitable for different multiplex PCR needs. It can be used for gel electrophoresis to identify multiple products of fragments of different sizes within 20 pairs, and can also be used for enrichment of hundreds of or more amplicons.
Features
- Easy to operate: ready-to-use 2× premixed solution
- High fidelity: 83 times the fidelity of Taq enzyme
- Long fragment amplification ability: high efficiency amplification within 40 kb
- Fast amplification speed: ＜10 kb, 5 sec/kb；≥10k, 10 sec/kb
- High sensitivity: amplify as low as 100 pg template
- Broad Compatibility : Suitable for complex samples, GC-rich/poor systems(20-80%), and multiplex amplification.
Applications
- Routine polymerase chain reaction (PCR)
- Multiplex PCR
- Fast PCR
- High-fidelity PCR
This kit is a ready-to-use 2× premixed solution containing High-Fidelity DNA Polymerase, dNTP s, and the buffer system carefully optimized for amplification of fragments within 20 Kb, with an extension rate of 10 sec/kb. Its fidelity is 83 times that of Taq DNA polymerase and 9 times that of Pfu DNA polymerase. It is suitable for complex template amplification, and the amplified product has a blunt end. This product is fast and simple, highly sensitive, specific and stable. It can amplify fragments with a GC content of 30% to 70%. It can amplify whole blood samples that have not been extracted with nucleic acids. It is suitable for blood preserved with EDTA, citrate or heparin, as well as dried blood spot cards, which is faster and more flexible. In addition, this product also contains a specific protective agent, so the premix can maintain stable activity after repeated freezing and thawing. All reagent components provided in this product have undergone strict quality inspection to ensure the excellent performance and batch-to-batch stability of the product to the highest extent. This reagent is suitable for different multiplex PCR needs. It can be used for gel electrophoresis to identify multiple products of fragments of different sizes within 20 pairs, and can also be used for enrichment of hundreds of or more amplicons.
Key Features
Easy to operate: ready-to-use 2× premixed solution High fidelity: 83 times the fidelity of Taq enzyme Long fragment amplification ability: high efficiency amplification within 40 kb Fast amplification speed: ＜10 kb, 5 sec/kb；≥10k, 10 sec/kb High sensitivity: amplify as low as 100 pg template Broad Compatibility : Suitable for complex samples, GC-rich/poor systems(20-80%), and multiplex amplification.
Kit Components
Components No. Name 17228ES08 (8 T) 17228ES24 (24 T) 17228ES96 (96 T) 17228ES97 (1000 T) 17228 2×Multiplex Long PCR Master Mix 200 μL 600 μL 2×1200 μL 25 mL
Storage & Shipping
Storage: -20°C | Shipping: Dry Ice
