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Description
Hieff NGS™ MaxUp rRNA Depletion Kit (Plant) is designed to remove rRNA (including cytoplasmic 18S and 25S rRNA, mitochondrial 18S and 26S rRNA, chloroplast 16S and 23S rRNA) from plant total RNA based on RNase H-based workflow and to retain mRNA and other non-coding RNA. This kit is suitable for both intact and partially degraded RNA samples.
Feature
- Strong Specificity: Efficiently removes rRNA from various plant samples
- Broad Input Compatibility: Suitable for a wide range of input amounts (10 ng to 1 μg).
- Consistent Quality: Strict batch-to-batch performance and stability controls ensure reliable results.
Specifications
| Depletion Technology | RNase H |
| Sample Type | Total RNA of Plant |
| Final Product Type | mRNA and other non coding RNAs |
| No. of Reactions | 24/96 Preps |
| Starting Material Amount | 100 ng~1 μg total RNA |
| Target | Remove rRNA |
Components
| Components No. | Name | 12254ES24 | 12254ES96 |
| 12254-A | Hybridization Buffer | 72 μL | 288 μL |
| 12254-B | Probe Mix (Plant) | 72 μL | 288 μL |
| 12254-C | RNase H Buffer | 72 μL | 288 μL |
| 12254-D | RNase H | 48 μL | 192 μL |
| 12254-E | DNase I Buffer | 660 μL | 2×1320 μL |
| 12254-F | DNase I | 60 μL | 240 μL |
Applications
rRNA Removal from Total RNA of Plant Roots, Seeds, and Leaves;
mRNA and Non-coding RNA Analysis
Storage
This product should be stored at -25~-15℃ for 12 months.
Figures
1. Efficient Removal of rRNA from Diverse Plant Species: This product has been verified for species including Arabidopsis, rice, wheat, cotton, corn, rapeseed, sunflower, tomato, walnut, strawberry, watermelon, barley, sweet potato, potato, mulberry, etc.
Figure 1. Efficient rRNA Removal using MaxUp rRNA Depletion Kit (Plant)
1 μg of total RNA from various plants was used to construct RNA libraries with the YEASEN Dual-mode RNA Library Prep Kit (Cat#12308) and rRNA Depletion Kit (Cat#12254). The percentage of rRNA reads before and after depletion was calculated to evaluate the rRNA removal efficiency.
2.Wide Compatibility with Different Input Amounts
Figure 2. Robust rRNA Removal Across Varying Input Amounts
Library preparation was performed using 12254 and RNA Library Preparation Kit (Cat#12308, with strand-specific protocols) on various plant sample types(Input RNA: 10 ng to 1 μg). The rRNA residual rate remained consistently low.
