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Hieff NGS™ OnePot Pro DNA Fragmentation Module provides a new-generation enzymatic fragmentation reagent for Illumina® and MGI® high-throughput sequencing platforms. The single-tube Smearase™ system performs simultaneous DNA fragmentation, end repair, and dA-tailing, eliminating intermediate cleanup steps. Fragmentation products proceed directly to adapter ligation using Hieff NGS™ Fast-Pace DNA Ligation Module (Cat# 12607) or Novel DNA Ligation Module (Cat# 12626). Supports 500 pg–1 µg dsDNA input with minimal GC bias.
Features
- Single-tube simultaneous fragmentation, end repair, and dA-tailing
- No intermediate cleanup steps before adapter ligation
- Broad input range: 500 pg to 1 µg dsDNA
- Minimal GC bias across animal, plant, and microbial genomes
- Compatible with Illumina® and MGI® sequencing platforms
- FFPE-compatible: tunable fragmentation time based on DIN score
Kit Components
| Component | 12619ES24 (24 rxn) | 12619ES96 (96 rxn) |
|---|---|---|
| Smearase™ Buffer (12619-A) | 240 µL | 960 µL |
| Smearase™ Enzyme (12619-B) | 120 µL | 480 µL |
FFPE Sample Fragmentation Guide
| Target Peak Size | Fragmentation Time | Recommended DIN |
|---|---|---|
| 250 bp | 9–13 min | >8.0 (high-quality FFPE) |
Applications
- NGS whole-genome library preparation (Illumina®, MGI®)
- FFPE sample NGS library preparation
- High-throughput library construction with minimal hands-on time
Enzymatic fragmentation (Smearase™) performs fragmentation at 37°C without mechanical stress, eliminating the need for specialized sonication equipment such as Bioruptor or Covaris systems. The single-tube format simultaneously performs fragmentation, end repair, and dA-tailing, replacing three separate workflow steps. Enzymatic fragmentation produces fragments with minimal GC bias compared to some mechanical methods.
500 pg to 1 µg of double-stranded DNA from animal, plant, or microbial genomes. For inputs below 50 ng, additional optimization of fragmentation time may be required to achieve the desired fragment size distribution.
Yes. The Smearase™ Fragmentation Module is specifically designed so that fragmentation products can proceed directly to adapter ligation using Hieff NGS™ Fast-Pace DNA Ligation Module (Cat# 12607) or Novel DNA Ligation Module (Cat# 12626) without an intermediate cleanup step. Skipping cleanup reduces total workflow time, minimizes DNA loss, and reduces cost per library.
For FFPE samples, fragmentation time is adjusted based on the DIN (DNA Integrity Number) score or the degree of degradation. Heavily degraded FFPE DNA (low DIN) requires shorter fragmentation times since the DNA is already partially fragmented by the fixation and storage process. For example, a target peak size of 250 bp requires 9–13 minutes for high-quality FFPE samples (DIN > 8.0).
Validated for use with Illumina® (NovaSeq, HiSeq, MiSeq, NextSeq) and MGI® (MGISEQ-2000, DNBSEQ) high-throughput sequencing platforms. Library preparation with this module generates fragments compatible with standard Illumina and MGI adapter sets.
Yeasen Biotechnology supports custom formulation and scale-up services for reagent mixes and master mixes. Contact BioHippo to discuss project requirements and initiate an inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen reagent mixes are available in GMP grade for IVD assay development. Manufactured in an ISO 13485-certified UCF.ME™ facility with FDA Drug Master File (DMF) support.
- GMP release testing and CoA documentation
- Scalable from milliliter to liter-scale quantities
- Consistent lot-to-lot performance specifications
▶ Glycerol-Free & Lyophilization-Ready Formats
Glycerol-free and lyophilization-ready formats are available for reagents intended for cartridge-based point-of-care devices, liquid handling automation, or ambient-temperature distribution.
- Glycerol-free liquid formulation
- Lyophilization-compatible format with cryoprotectant
- Custom reaction buffer optimization
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting team provides end-to-end support for molecular diagnostic product development using Yeasen reagent mixes as a foundation.
- Master mix selection and performance matching for target assay
- Primer/probe design and assay buffer optimization
- Sensitivity, specificity, and LOD validation
- Instrument compatibility assessment
▶ Custom Formulation
Custom formulation services are available for researchers requiring modifications to standard reagent mix compositions, including ROX level adjustment, dye substitution, stabilizer optimization, and buffer modification.
- ROX concentration customization (No / Low / High ROX)
- Reference dye and indicator dye options
- Buffer composition and pH optimization
- Scale-up to commercial quantities on confirmation
ⓘ Customization services fulfilled by Yeasen Biotechnology. Contact BioHippo for project scoping and pricing.
