| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Hieff™ Taq DNA polymerase is a thermostable recombinant wild type DNA polymerase expressed by Thermus aquaticus with a molecular weight of 94 KDa. It has 5'→3' polymerase activity and 5'→3' exonuclease activity but without 3'→5' exonuclease activity. The amplified product has 3'- dA and can be directly used for TA cloning.
Features
- 5'→3' DNA polymerase activity
- 5'→3' exonuclease activity
- Hieff™ Taq DNA polymerase is an ideal tool for standard PCR with templates of 5 kb or less
Applications
Genotyping, colony PCR and other conventional PCR
Specifications
| Polymerase | Taq DNA polymerase |
| Molecular Weight | 94 KDa |
| Unit Definition | Using the activated DNA of salmon sperm as template/primer, the activity is defined as one active unit (U) when 10 nmol of total nucleotide was ingested as acid insoluble substance at 74℃ for 30 min. |
Glycerol Content |
Contains Glycerol |
Components
| Components No. | Name | 10101ES80 (1, 000 U) | 10101ES92 (10, 000 U) |
| 10101-A | 10×Taq Buffer (Mg2+ Free) | 4×1 mL | 4×10 mL |
| 10101-B | 25 mM MgCl2 | 2×1 mL | 2×10 mL |
| 10101-C | Hieff™ Taq DNA Polymerase (5 U/μL) | 200 μL | 2×1 mL |
Storage
The product should be stored at -25℃~-15℃ for two years.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
Genotyping, colony PCR and other conventional PCR. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of deoxyribonucleotide into acid-insoluble material in 30 min at 74°C using activated salmon sperm DNA as template/primer.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Standard Taq DNA polymerase lacks 3′→5′ proofreading exonuclease activity, resulting in an error rate of approximately 1 in 10⁵ bp per cycle. It produces predominantly A-tailed PCR products, compatible with TA cloning. Typical routine amplification yields fragments up to ~5 kb from genomic DNA and up to ~10 kb from plasmid/cDNA templates under optimized conditions.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
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The Double-Block anti-Taq DNA Polymerase Antibody to Help Reducing Nonspecific Amplification
