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Hieff UNICON™ ColorGPS qPCR Master Mix (High ROX) is a 2× real-time quantitative PCR premix featuring high fluorescence intensity, sensitivity, specificity, and amplification yield. Antibody-based Hot Start inhibits non-specific amplification. ColorGPS mixed-dye pipetting tracking reduces pipetting errors. This High ROX variant provides a high-concentration ROX passive reference signal compatible with instruments requiring High ROX correction (e.g., Applied Biosystems systems).
Features
- ColorGPS pipetting tracking: color-change dye verifies pipetting accuracy
- Antibody-based Hot Start: inhibits non-specific amplification
- High ROX: compatible with Applied Biosystems 7500, 7900HT, QuantStudio, and similar instruments
- High fluorescence intensity and amplification yield
- Low inter-well variability: improved replicate reproducibility
The High ROX format is designed for Applied Biosystems instruments including the 7500, 7500 Fast, 7900HT, QuantStudio 3/5/6/7, ViiA™7, StepOne™, and StepOnePlus™. These platforms require a high-concentration ROX passive reference signal for baseline correction algorithms to function correctly.
ColorGPS uses a mixed-dye color-change reaction triggered by dispensing the master mix. The visible color change in each well provides immediate confirmation that the master mix was correctly added, enabling rapid visual quality check of the entire plate before template addition.
Yes. This formulation is validated for both standard 2-hour and rapid 40-minute qPCR cycling programs, providing flexibility to optimize run time on Applied Biosystems Fast-mode instruments.
Store at −25 to −15°C. Shipped on dry ice. Stable for the stated shelf life under recommended storage conditions.
Valid results: amplification efficiency 90–110%; R² > 0.98; S-shaped curve with Ct 15–35; single-peak melting curve; NTC Ct > 35.
Yeasen Biotechnology supports custom formulation and scale-up services for reagent mixes and master mixes. Contact BioHippo to discuss project requirements and initiate an inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen reagent mixes are available in GMP grade for IVD assay development. Manufactured in an ISO 13485-certified UCF.ME™ facility with FDA Drug Master File (DMF) support.
- GMP release testing and CoA documentation
- Scalable from milliliter to liter-scale quantities
- Consistent lot-to-lot performance specifications
▶ Glycerol-Free & Lyophilization-Ready Formats
Glycerol-free and lyophilization-ready formats are available for reagents intended for cartridge-based point-of-care devices, liquid handling automation, or ambient-temperature distribution.
- Glycerol-free liquid formulation
- Lyophilization-compatible format with cryoprotectant
- Custom reaction buffer optimization
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting team provides end-to-end support for molecular diagnostic product development using Yeasen reagent mixes as a foundation.
- Master mix selection and performance matching for target assay
- Primer/probe design and assay buffer optimization
- Sensitivity, specificity, and LOD validation
- Instrument compatibility assessment
▶ Custom Formulation
Custom formulation services are available for researchers requiring modifications to standard reagent mix compositions, including ROX level adjustment, dye substitution, stabilizer optimization, and buffer modification.
- ROX concentration customization (No / Low / High ROX)
- Reference dye and indicator dye options
- Buffer composition and pH optimization
- Scale-up to commercial quantities on confirmation
ⓘ Customization services fulfilled by Yeasen Biotechnology. Contact BioHippo for project scoping and pricing.
