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Hieff UNICON™ ColorGPS qPCR Master Mix (Low ROX) is a 2× real-time quantitative PCR premix featuring high fluorescence intensity, sensitivity, specificity, and amplification yield. Antibody-based Hot Start inhibits non-specific amplification. ColorGPS mixed-dye pipetting tracking reduces pipetting errors. This Low ROX variant provides a low-concentration ROX passive reference signal compatible with instruments requiring Low ROX correction (e.g., Bio-Rad CFX, Roche LightCycler 480).
Features
- ColorGPS pipetting tracking: color-change dye verifies pipetting accuracy
- Antibody-based Hot Start: inhibits non-specific amplification
- Low ROX: compatible with Bio-Rad CFX, Roche LightCycler 480, and similar instruments
- High fluorescence intensity and amplification yield
- Low inter-well variability: improved replicate reproducibility
The Low ROX format is optimized for instruments that require a low-concentration ROX passive reference signal, including Bio-Rad CFX96, CFX384, Roche LightCycler 480, and similar platforms. Using High ROX on these instruments can saturate the reference channel, while using No ROX on platforms expecting ROX can affect baseline correction.
ColorGPS uses a mixed-dye color-change mechanism that provides visual confirmation of master mix dispensing into each well. This allows the operator to visually scan the plate for any wells that appear colorless (missed) or double-colored (double-dispensed) before adding template and proceeding.
This mix demonstrates high fluorescence intensity with low inter-well variability, accurate template concentration resolution, and compatibility with both standard and rapid qPCR cycling programs, providing flexibility for different instrument speed configurations.
Store at −25 to −15°C. Shipped on dry ice. For long-term stability, store as supplied and avoid repeated freeze-thaw of working aliquots.
Valid results: amplification efficiency 90–110%; R² > 0.98; S-shaped curve with Ct 15–35; single-peak melting curve; NTC Ct > 35.
Yeasen Biotechnology supports custom formulation and scale-up services for reagent mixes and master mixes. Contact BioHippo to discuss project requirements and initiate an inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen reagent mixes are available in GMP grade for IVD assay development. Manufactured in an ISO 13485-certified UCF.ME™ facility with FDA Drug Master File (DMF) support.
- GMP release testing and CoA documentation
- Scalable from milliliter to liter-scale quantities
- Consistent lot-to-lot performance specifications
▶ Glycerol-Free & Lyophilization-Ready Formats
Glycerol-free and lyophilization-ready formats are available for reagents intended for cartridge-based point-of-care devices, liquid handling automation, or ambient-temperature distribution.
- Glycerol-free liquid formulation
- Lyophilization-compatible format with cryoprotectant
- Custom reaction buffer optimization
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting team provides end-to-end support for molecular diagnostic product development using Yeasen reagent mixes as a foundation.
- Master mix selection and performance matching for target assay
- Primer/probe design and assay buffer optimization
- Sensitivity, specificity, and LOD validation
- Instrument compatibility assessment
▶ Custom Formulation
Custom formulation services are available for researchers requiring modifications to standard reagent mix compositions, including ROX level adjustment, dye substitution, stabilizer optimization, and buffer modification.
- ROX concentration customization (No / Low / High ROX)
- Reference dye and indicator dye options
- Buffer composition and pH optimization
- Scale-up to commercial quantities on confirmation
ⓘ Customization services fulfilled by Yeasen Biotechnology. Contact BioHippo for project scoping and pricing.
