| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Hieff UniconTM Hotstart Direct Taq DNA Polymerase is a hot-start DNA polymerase that tolerates blood and other inhibitors. This product uses antibody blocking and has good amplification sensitivity and specificity. This product can be completely inactivated by heating the blocking antibody at the pre-denaturation temperature for 30 sec, releasing the DNA polymerase activity. The use of this hot-start Taq enzyme can effectively inhibit amplification caused by nonspecific annealing of primers.
Features
Enhanced inhibitor tolerance: The qPCR Mix prepared with D-taq exhibits superior resistance to common PCR inhibitors such as EDTA, sodium citrate, and whole blood.
Nuclease-free: Free from residual exonucleases, endonucleases, and RNase activity.
High sensitivity and specificity: Ideal for PCR applications requiring precise and sensitive detection.
Excellent stability: Maintains consistent performance after storage at 4°C or 37°C for 7 days.
Components
|
Components No. |
Name |
10717ES72 |
10717ES76 |
10717ES80 |
10717ES92 |
|
10717 |
Hotstart D-Taq (5 U/μL) |
50 μL |
100 μL |
200 μL |
1 mL×2 |
Shipping and Storage
This product should be stored at -25~-15℃ for 2 years.
Application
hot-start PCR and qPCR; RT-qPCR Detection;
Diagnostic pathogen detection (respiratory tract, intestinal tract, etc.); animal pathogen detection (non-swine fever, etc.); food testing (microorganisms, etc.)
Figures
1. Enhanced inhibitor tolerance:
Figure 1. Comparison of Amplification Efficiency with Various Inhibitors
(a) blood-derived inhibitors (EDTA, sodium citrate):
(b) 2% plasma with pseudovirus or standard template (5 μL in 25 μL reaction).
(c) 2% plasma with pseudovirus or standard template (5 μL in 25 μL reaction).
2. Ultra-high sensitivity: down to 3 copies per reaction.
Figure 2. Sensitivity test
Using ASFV primer probe, 20 replicate wells were tested on ASFV single plasmid (3 copies of the final system), and the detection rates of reagents of various brands were compared. The results showed that Yeasen products had a higher detection rate.
3. Stability Test
Figure 3. Real-time stability testing(A) and Freeze-thaw stability(B)
qPCR premix prepared with 10717 DNA polymerase was tested for accelerated stability (37°C for 7 and 14 days) and freeze-thaw stability (-20°C, repeated cycles) using ASFV plasmid as template. Ct and fluorescence values remained consistent, indicating stable performance.
Glycerol Content: Contains Glycerol
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in PCR & Amplification, Molecular Diagnostics applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
One unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide in 30 min at 74°C. The Hot Start format employs monoclonal antibody or chemical modification to inhibit polymerase activity below the denaturation temperature.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Hot Start formulations use monoclonal antibodies or chemical modification to block polymerase activity at room temperature, preventing non-specific extension during reaction setup. Full activity is restored after initial denaturation (≥95°C). This results in reduced primer-dimer formation, suppression of non-specific amplification products, and improved sensitivity in complex or low-abundance templates.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
