| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Hieff UNICONTM HotStart E-Taq DNA Polymerase, Glycerol-free is a hot start DNA polymerase with double blocking by double antibodies independently developed by the company. This product not only blocks the 5'→3' polymerase activity of Taq DNA polymerase, but also blocks the 5'→3' exonuclease activity. Heating for 30 seconds at the pre denaturation temperature can completely inactivate the antibody and release DNA polymerase activity and exonuclease activity. The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation, so as to make the in vitro detection reagent more stable during transportation or use at room temperature. This product contains no Glycerol and is specially designed for the preparation of lyophilized reagents.
Specifications
|
Cat.No. |
14316ES76 / 14316ES80 / 14316ES92 / 14316ES97 / 14316ES98 |
|
Size |
500 U / 1000 U / 10 KU / 50 KU / 100 KU |
Components
|
Name |
14316ES76 |
14316ES80 |
14316ES92 |
|
Hieff UNICONTM HotStart E-Taq DNA Polymerase, Glycerol-free (5 U/μL) |
100 μL |
200 μL |
2×1 mL |
Figure
Storage
This product should be stored at 2~8℃ for 1 years.
Instructions
1.Reaction Setup
|
Components |
Volume (μL) |
Final Concentration |
|
2×Buffer |
25 |
1× |
|
Primer/Probe mix |
X |
0.1 μmol/L-0.5 μmol/L |
|
Hieff UNICONTM HotStart E-Taq DNA Polymerase, Glycerol-free (5 U/μL) |
1.2 |
0.12 U/μL |
|
DNA template |
X |
0.1-100 ng |
|
ddH2O |
up to 50 |
- |
*According to the specific experimental application, the corresponding reaction Buffer should be prepared by oneself. The amount of DNA and primer concentration in the above table are recommended concentrations, and the optimal concentration can be adjusted according to the specific experimental situation.
- Thermal cycling protocol (2-Step cycling protocol)
|
Stage |
Temperature |
Time |
Cycles |
|
Pre-denaturation |
95℃ |
5 min |
1 |
|
Denaturation |
95℃ |
15 sec |
45 |
|
Annealing/Extension |
60℃ |
30 sec |
*The reaction temperature is adjusted according to the Tm value of the designed primers. Different qPCR instruments need different fluorescence signal acquisition time, please set according to the shortest time limit.
Notes
1.This product is for scientific research purposes only.
2.Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety.
Documents:
Manuals
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in PCR & Amplification, Molecular Diagnostics applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
One unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide in 30 min at 74°C. The Hot Start format employs monoclonal antibody or chemical modification to inhibit polymerase activity below the denaturation temperature.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. The formulation is glycerol-free, making it compatible with lyophilization, high-throughput liquid handling systems, and direct integration into IVD master mix formulations. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Hot Start formulations use monoclonal antibodies or chemical modification to block polymerase activity at room temperature, preventing non-specific extension during reaction setup. Full activity is restored after initial denaturation (≥95°C). This results in reduced primer-dimer formation, suppression of non-specific amplification products, and improved sensitivity in complex or low-abundance templates.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
