| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Hieff Unicon™ Hotstart J-Taq DNA Polymerase, Glycerol-free (40 U/μL) is a hot-start DNA polymerase. This product utilizes antibody-mediated inhibition, providing high amplification sensitivity and specificity. The inhibitory antibody is completely inactivated and the DNA polymerase activity is fully released after a 30-second heat activation step at the initial denaturation temperature. Using this hot-start Taq enzyme effectively suppresses non-specific amplification caused by primer mis-annealing. This glycerol-free formulation is specifically designed for lyophilized reagent preparation.
Features
Rapid Activation: Taq polymerase achieves full activity after a quick 30 s incubation at 95 °C, ensuring immediate and efficient amplification.
High Sensitivity & Specificity: Provides strong amplification performance even with low template input, minimizing non-specific products.
Broad Instrument Compatibility: Works seamlessly with a wide range of standard PCR and real-time PCR platforms.
Lyophilization-Ready: Formulation supports freeze-drying, enabling long-term storage, reduced shipping costs, and simplified reagent handling.
Components
|
Name |
14326ES02 |
14326ES20 |
14326ES30 |
|
Hotstart J-Taq DNA Polymerase, Glycerol-free ( 40 U/μL) |
50 μL |
500 μL |
2×1 mL |
Storage
This product should be stored at -25~-15℃ for 1 year.
Application
Gene Expression Analysis; Low-Copy Target Amplification; Lyophilized PCR/qPCR.
Figures
1. Consistent Performance & Strong Stability
Figure 1. Stability of Yeasen glycerol-free enzyme (14326ES).
The glycerol-
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in PCR & Amplification, Molecular Diagnostics applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
One unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide in 30 min at 74°C. The Hot Start format employs monoclonal antibody or chemical modification to inhibit polymerase activity below the denaturation temperature.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. The formulation is glycerol-free, making it compatible with lyophilization, high-throughput liquid handling systems, and direct integration into IVD master mix formulations. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Hot Start formulations use monoclonal antibodies or chemical modification to block polymerase activity at room temperature, preventing non-specific extension during reaction setup. Full activity is restored after initial denaturation (≥95°C). This results in reduced primer-dimer formation, suppression of non-specific amplification products, and improved sensitivity in complex or low-abundance templates.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
