| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Hieff UNICONTM HotStart Taq DNA Polymerase is a mixture of Hieff UNICONTM Taq Antibody(Catalog #31301ES)and HieffTM Taq DNA Polymerase. Hieff UNICONTM Taq Antibody had a high affinity for HieffTM Taq DNA Polymerase, and the activity of HieffTM Taq DNA Polymerase could still be blocked after being treated at 50℃ for 30 min. The product was completely inactivated by heating at pre denaturation temperature for 30 seconds, releasing DNA polymerase activity. The hot start Taq enzyme can effectively inhibit the amplification caused by non-specific annealing of primers.
Hieff UNICONTM HotStart Taq DNA Polymerase is suitable for hot start PCR and qPCR. HieffTM Taq DNA Polymerase in this product is a heat stable recombinant DNA polymerase with high template affinity, which is very suitable for amplification of low copy template. The amplified product has 3 '- dA and can be easily cloned into T vector.
Features
- Closed Detection of DNA Polymerase Activity：In the Hieff UNICONTM HotStart Taq Buffer (Mg2+ Plus) ，the activity release was less than 5% after incubation at 65℃ for 30 min.
- DNA Polymerase Activity Release Detection ：In the HieffUNICONTM HotStart Taq Buffer (Mg2+ Plus) ，the activity release was higher than 95% when heated at 95℃ for 30 sec.
- Exonuclease Activity: In 20 μL reaction system, 10 U of this product and 0.6 μg of λ - HindIII were incubated at 74℃ for 1 h, and the electrophoresis band of DNA had no change.
- Endonuclease Activity: In 20 μL reaction system, 10 U of the product and 0.6 μg of supercoiled pBR322 DNA were incubated at 74 ℃ for 1 h, and the DNA electrophoretic bands did not change.
- Residual E.coli gDNA Test: The E.coil 16S rDNA gene was amplified by adding 2 U of the product into 50 μL system and using sterile ddH2O as template. After 35 cycles, the amplified products were subjected to 1% agarose gel electrophoresis and EB staining without amplification bands.
Specifications
|
Polymerase |
Taq DNA Polymerase |
|
Purity |
≥ 95%（SDS-PAGE） |
|
Hot Start |
Built-In Hot Start |
|
Reaction Speed |
Standard |
|
Exonuclease Activity |
5' - 3' |
|
Unit Definition |
Using the activated DNA of salmon sperm as template / primer, the activity was defined as one active unit (U) when 10 nmol of total nucleotide was ingested as acid insoluble substance at 74℃ for 30 min. |
Glycerol Content |
Contains Glycerol |
Components
|
Name |
10730ES00 (500 U) |
10730ES01 (5,000 U) |
10730ES03 (50,000 U) |
10730ES25 (1,250,000 U) |
|
HotStart Taq DNA Polymerase (50 U/μL) |
10 μL |
100 μL |
1 mL |
25 mL |
Storage
This product should be stored at -25~-15℃ for 2 years.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in PCR & Amplification, Molecular Diagnostics applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
One unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide in 30 min at 74°C. The Hot Start format employs monoclonal antibody or chemical modification to inhibit polymerase activity below the denaturation temperature.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Hot Start formulations use monoclonal antibodies or chemical modification to block polymerase activity at room temperature, preventing non-specific extension during reaction setup. Full activity is restored after initial denaturation (≥95°C). This results in reduced primer-dimer formation, suppression of non-specific amplification products, and improved sensitivity in complex or low-abundance templates.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
