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Hieff UNICON™ Lyo-Ready I TaqMan Multiplex qPCR Mix (UDG Plus) is a fluorescent quantitative PCR reagent based on a new-generation antibody-based Hot Start Fast Taq DNA Polymerase, designed for lyophilization-compatible IVD formats. Contains lyophilization protectant, enabling direct freeze-drying without reformulation. Integrated UDG (Uracil-DNA Glycosylase) activity prevents aerosol carry-over contamination, critical for clinical diagnostic applications.
Features
- Lyophilization-ready: direct freeze-drying without reformulation
- UDG Plus: integrated UDG prevents aerosol carry-over contamination
- Antibody-based Hot Start Fast Taq: rapid activation, high specificity
- Dual-use: liquid reagent or lyophilized long-term storage format
- Probe compatibility: FAM, HEX, TET, JOE, ROX, and Molecular Beacon probes
- No ROX: for instruments not requiring ROX passive reference
Applications
- Multiplex TaqMan probe-based qPCR
- Lyophilized point-of-care diagnostic assay development
- IVD master mix formulation
- Carryover contamination-controlled quantitative PCR
Lyophilization (freeze-drying) enables conversion of liquid qPCR reagents into stable, room-temperature-storable dry formats for point-of-care diagnostic cartridges and decentralized testing devices. A lyophilization-ready mix contains specific cryoprotectants that maintain enzyme activity, dNTP integrity, and buffer composition through the freeze-drying and rehydration cycle without performance loss.
Uracil-DNA Glycosylase (UDG) degrades any dU-containing DNA carryover from previous reactions. When dUTP is substituted for dTTP in the PCR reaction, all amplification products contain dU. Before each new run, UDG is active at lower temperatures to digest any carryover amplicon. UDG is inactivated during the initial denaturation step, allowing normal amplification of native (dT-containing) target DNA to proceed. This is a critical contamination control for high-throughput diagnostic laboratories.
Compatible with dual-labeled probes including FAM, HEX, TET, JOE, and ROX fluorophores, as well as Molecular Beacon probes. This broad compatibility allows use across diverse multiplex panel designs with different probe chemistries.
No. This mix does not contain ROX passive reference dye. It is designed for instruments where ROX correction is not required or where the ROX channel is reserved for a probe fluorophore in the multiplex panel.
Lyophilized reagent preparation stability depends on the specific lyophilization protocol and cartridge format, and should be validated by the user for their specific application. As a liquid reagent, this mix is stable at −20°C under standard storage conditions.
Yeasen Biotechnology supports custom formulation and scale-up services for reagent mixes and master mixes. Contact BioHippo to discuss project requirements and initiate an inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen reagent mixes are available in GMP grade for IVD assay development. Manufactured in an ISO 13485-certified UCF.ME™ facility with FDA Drug Master File (DMF) support.
- GMP release testing and CoA documentation
- Scalable from milliliter to liter-scale quantities
- Consistent lot-to-lot performance specifications
▶ Glycerol-Free & Lyophilization-Ready Formats
Glycerol-free and lyophilization-ready formats are available for reagents intended for cartridge-based point-of-care devices, liquid handling automation, or ambient-temperature distribution.
- Glycerol-free liquid formulation
- Lyophilization-compatible format with cryoprotectant
- Custom reaction buffer optimization
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting team provides end-to-end support for molecular diagnostic product development using Yeasen reagent mixes as a foundation.
- Master mix selection and performance matching for target assay
- Primer/probe design and assay buffer optimization
- Sensitivity, specificity, and LOD validation
- Instrument compatibility assessment
▶ Custom Formulation
Custom formulation services are available for researchers requiring modifications to standard reagent mix compositions, including ROX level adjustment, dye substitution, stabilizer optimization, and buffer modification.
- ROX concentration customization (No / Low / High ROX)
- Reference dye and indicator dye options
- Buffer composition and pH optimization
- Scale-up to commercial quantities on confirmation
ⓘ Customization services fulfilled by Yeasen Biotechnology. Contact BioHippo for project scoping and pricing.
