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Hieff UNICON™ qPCR TaqMan Probe Master Mix is a 2× premix for probe-based real-time quantitative PCR. The antibody-based Hot Start UNICON™ Taq DNA Polymerase inhibits non-specific amplification during sample preparation. Optimized buffer additives enhance amplification efficiency and sensitivity for low-concentration templates.
Features
- Antibody-based Hot Start: prevents non-specific amplification
- High sensitivity: suitable for low-copy-number template quantification
- High specificity: probe-based detection discriminates single-base differences
- Broad instrument compatibility
Specifications
| Parameter | Value |
|---|---|
| Hot Start | Antibody-based |
| Detection Method | Probe-Based (TaqMan) |
| PCR Method | qPCR / RT-qPCR |
| Sample Type | DNA / cDNA / RNA |
Compatible Instruments
Applied Biosystems (5700, 7000, 7300, 7700, 7900HT Fast, StepOne™, StepOnePlus™, 7500, 7500 Fast, ViiA™7, QuantStudio™ 3/5/6/7/12k Flex); Bio-Rad (CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Chromo4); Eppendorf (Mastercycler ep realplex, realplex 2s); Qiagen Corbett (Rotor-Gene Q, 3000, 6000); Roche (LightCycler 480, 2.0, 96); Stratagene (MX3000P™, MX3005P™, MX4000P™); Thermo PikoReal; Cepheid SmartCycler; Illumina Eco qPCR.
Selected Citations
[1] Hou W, et al. c-Myc participates in high glucose-mediated endothelial inflammation via upregulation of IRAK1 expression in diabetic nephropathy. Cell Signal. 2022;92:110263. (IF: 4.315)
TaqMan probe-based detection provides a second layer of sequence-specific verification beyond primer annealing — the probe must hybridize to its complementary target sequence within the amplicon for signal to be generated. This eliminates false-positive signal from primer dimers and non-specific amplification products, providing higher specificity. Probe-based detection is also essential for multiplex applications using multiple probes with different fluorophores.
UNICON™ Taq DNA Polymerase uses antibody-based Hot Start, blocking polymerase activity at room temperature and releasing full activity upon initial denaturation at ≥95°C. This is particularly important for TaqMan qPCR with low-abundance targets, where any non-specific amplification during setup reduces assay sensitivity.
Valid results require: amplification efficiency 90–110%; R² > 0.98 for standard curves; S-shaped amplification curve with Ct between 15 and 35; NTC Ct > 35. Ct values below 15 may compress the dynamic range.
Compatible with Applied Biosystems (5700, 7000, 7300, 7700, 7900HT Fast, StepOne, StepOnePlus, 7500, 7500 Fast, ViiA7, QuantStudio 3/5/6/7/12k Flex), Bio-Rad (CFX96, CFX384, iCycler iQ, iQ5, MyiQ), Eppendorf (Mastercycler ep realplex), Qiagen Corbett (Rotor-Gene Q), Roche (LightCycler 480, 2.0, 96), Stratagene, Thermo PikoReal, Cepheid SmartCycler, and Illumina Eco qPCR.
Yes. The optimized buffer additives enhance amplification efficiency and sensitivity for low-concentration templates, making this mix suitable for detection of low-copy targets in complex sample matrices including clinical specimens.
Yeasen Biotechnology supports custom formulation and scale-up services for reagent mixes and master mixes. Contact BioHippo to discuss project requirements and initiate an inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen reagent mixes are available in GMP grade for IVD assay development. Manufactured in an ISO 13485-certified UCF.ME™ facility with FDA Drug Master File (DMF) support.
- GMP release testing and CoA documentation
- Scalable from milliliter to liter-scale quantities
- Consistent lot-to-lot performance specifications
▶ Glycerol-Free & Lyophilization-Ready Formats
Glycerol-free and lyophilization-ready formats are available for reagents intended for cartridge-based point-of-care devices, liquid handling automation, or ambient-temperature distribution.
- Glycerol-free liquid formulation
- Lyophilization-compatible format with cryoprotectant
- Custom reaction buffer optimization
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting team provides end-to-end support for molecular diagnostic product development using Yeasen reagent mixes as a foundation.
- Master mix selection and performance matching for target assay
- Primer/probe design and assay buffer optimization
- Sensitivity, specificity, and LOD validation
- Instrument compatibility assessment
▶ Custom Formulation
Custom formulation services are available for researchers requiring modifications to standard reagent mix compositions, including ROX level adjustment, dye substitution, stabilizer optimization, and buffer modification.
- ROX concentration customization (No / Low / High ROX)
- Reference dye and indicator dye options
- Buffer composition and pH optimization
- Scale-up to commercial quantities on confirmation
ⓘ Customization services fulfilled by Yeasen Biotechnology. Contact BioHippo for project scoping and pricing.
