| Field | Specification |
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Hieff UNICON™ Universal Probe Multiplex qPCR Master Mix enables up to four simultaneous fluorescent quantitative PCR reactions in a single well. The genetically modified antibody-based Hot Start Taq enzyme provides improved amplification sensitivity and specificity. A deeply optimized multiplex reaction buffer enhances amplification efficiency for low-concentration templates. Compatible with FAM, HEX, ROX, and other fluorescent probe channels.
Features
- Up to 4-plex probe-based qPCR in a single well
- Antibody-based Hot Start: enhanced specificity and sensitivity
- Strong tolerance to blood-derived inhibitors
- Blue color indicator for visual pipetting tracking
- Super storage stability: thermal acceleration and freeze-thaw validated
- Compatible with all mainstream qPCR instruments
- Stable for 2 years at −15 to −25°C
Applications
- Multiplex pathogen detection
- Copy number variation (CNV) analysis
- SNP genotyping
- Quantitative gene expression profiling
Reaction Setup
| Component | Volume (µL) | Final Concentration |
|---|---|---|
| 2× Hieff UNICON™ Universal TaqMan Multiplex Master Mix | 12.5 | 1× |
| Primer Mix (10 µmol/L) | x | 0.1–0.5 µmol/L |
| Probe Mix (10 µmol/L) | x | 50–250 nmol/L |
| ROX Reference Dye (optional) | 0.4 | 1× |
| Template DNA/cDNA | 1–10 | — |
| ddH₂O | To 25 | — |
Cycling Conditions
| Step | Temperature | Time | Cycles |
|---|---|---|---|
| Initial Denaturation | 95°C | 5 min | 1 |
| Denaturation | 95°C | 15 sec | 40–45 |
| Annealing/Extension | 60°C | 30 sec | 40–45 |
Selected Citations
[1] Zhang P, et al. Transcriptomic and metabolomic profiling of LED light quality on fruit ripening in Cabernet Sauvignon grape. Front Nutr. 2021;8:790697.
[2] Xu B, et al. Histone methyltransferase Dot1L recruits O-GlcNAc transferase to target chromatin sites. J Biol Chem. 2022;298(7):102115.
This mix supports up to 4-plex detection — up to four different probe-based targets in a single reaction well using spectrally distinct fluorophores (e.g., FAM, HEX, ROX, Cy5). Each probe must have a distinct excitation/emission spectrum detectable by the qPCR instrument fluorescence channels.
This formulation has strong tolerance to common blood-derived inhibitors such as hemoglobin, IgG, and heparin. This allows use of minimally processed clinical samples including extracted DNA from blood, serum, and plasma without additional purification steps beyond standard extraction.
Compatible with standard dual-labeled TaqMan probes (5′ fluorophore + 3′ quencher), including FAM, HEX, ROX, Cy5, and other dual-labeled formats. Molecular Beacon probes are also compatible.
Stable for 2 years at −15 to −25°C. Shipped on dry ice. Freeze-thaw stability validated to 50 cycles. Thermal acceleration stability validated at 37°C for 14 days without significant performance change.
Valid results: amplification efficiency 90–110% per channel; R² > 0.98 per channel; S-shaped amplification curve with Ct 15–35 per channel; NTC Ct > 35 for each target; no cross-channel spectral bleed-through exceeding instrument correction capability.
Yeasen Biotechnology supports custom formulation and scale-up services for reagent mixes and master mixes. Contact BioHippo to discuss project requirements and initiate an inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen reagent mixes are available in GMP grade for IVD assay development. Manufactured in an ISO 13485-certified UCF.ME™ facility with FDA Drug Master File (DMF) support.
- GMP release testing and CoA documentation
- Scalable from milliliter to liter-scale quantities
- Consistent lot-to-lot performance specifications
▶ Glycerol-Free & Lyophilization-Ready Formats
Glycerol-free and lyophilization-ready formats are available for reagents intended for cartridge-based point-of-care devices, liquid handling automation, or ambient-temperature distribution.
- Glycerol-free liquid formulation
- Lyophilization-compatible format with cryoprotectant
- Custom reaction buffer optimization
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting team provides end-to-end support for molecular diagnostic product development using Yeasen reagent mixes as a foundation.
- Master mix selection and performance matching for target assay
- Primer/probe design and assay buffer optimization
- Sensitivity, specificity, and LOD validation
- Instrument compatibility assessment
▶ Custom Formulation
Custom formulation services are available for researchers requiring modifications to standard reagent mix compositions, including ROX level adjustment, dye substitution, stabilizer optimization, and buffer modification.
- ROX concentration customization (No / Low / High ROX)
- Reference dye and indicator dye options
- Buffer composition and pH optimization
- Scale-up to commercial quantities on confirmation
ⓘ Customization services fulfilled by Yeasen Biotechnology. Contact BioHippo for project scoping and pricing.
