HIF1A Antibody

SKU:BHA10556389
Overview
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HIF1A-specific polyclonal antibody raised in Rabbit. Validated for ELISA, Western Blot in Human, Mouse, Rat samples. Affinity-purified.
Format Unconjugated
Immunogen Native Protein
Applications 2 validated
Dilution ELISA:1:2000-1:5000, WB:1:500-1:2000
Target Aliases 34 aliases
Options selector
Catalog no. Size
CSB-PA928186-50UL 50 uL
CSB-PA928186-100UL 100 uL
Available Options
Size
Field Specification
Target HIF1A
Antigen
  • Synthetic peptide of Human HIF1A
Alternative names ARNT interacting protein; ARNT-interacting protein; Basic helix loop helix PAS protein MOP1; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF 1alpha; HIF-1-alpha; HIF-1alpha; HIF-alpha; HIF1 A; HIF1 Alpha; HIF1; HIF1-alpha; HIF1A; HIF1A_HUMAN; hifla; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3 ; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Hypoxia inducible factor 1; alpha subunit (basic helix loop helix transcription factor); Hypoxia inducible factor1alpha; Hypoxia-inducible factor 1-alpha; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; MOP1; PAS domain-containing protein 8; PASD 8; PASD8
UniProt # Q16665
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • ELISA
  • Western Blot
Immunogen Synthetic peptide of Human HIF1A
Conjugate
  • Non-conjugated
Source This product is a polyclonal antibody purified from rabbit antiserum.
Purification Antigen affinity purification
Form Liquid
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Storage buffer PBS, 0.05% sodium azide, 40% glycerol, pH 7.4.
Catalog no. (Mfr.) CSB-PA928186
Main SKU BHA10556389

Product Overview

Primary Antibody

This antibody targets HIF1A and was raised against a recombinant protein immunogen.

Validated Applications

Application Recommended Dilution
ELISA ELISA:1:2000-1:5000, WB:1:500-1:2000
Western Blot (WB) ELISA:1:2000-1:5000, WB:1:500-1:2000
✔ Validated by CUSABIO Technology

Antibody Specifications

Target HIF1A
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Purification Affinity Purification
Antigen / Immunogen Synthetic peptide of Human HIF1A
Production This product is a polyclonal antibody purified from rabbit antiserum.
Form Liquid
Storage Buffer PBS, 0.05% sodium azide, 40% glycerol, pH 7.4.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names

ARNT interacting protein; ARNT-interacting protein; Basic helix loop helix PAS protein MOP1; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF 1alpha; HIF-1-alpha; HIF-1alpha; HIF-alpha; HIF1 A; HIF1 Alpha; HIF1; HIF1-alpha; HIF1A; HIF1A_HUMAN; hifla; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3 ; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Hypoxia inducible factor 1; alpha subunit (basic helix loop helix transcription factor); Hypoxia inducible factor1alpha; Hypoxia-inducible factor 1-alpha; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; MOP1; PAS domain-containing protein 8; PASD 8; PASD8

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle with standard laboratory precautions. Dispose per local regulations.
TargetHIF1A
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ConjugateUnconjugated
ReactivityHuman, Mouse, Rat
PurificationAffinity Purification
Antigen/ImmunogenSynthetic peptide of Human HIF1A
ProductionThis product is a polyclonal antibody purified from rabbit antiserum.
FormLiquid
Storage BufferPBS, 0.05% sodium azide, 40% glycerol, pH 7.4.
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Dilutions are starting recommendations. Titration is advised for each system.

ApplicationRecommended Dilution
ELISAELISA:1:2000-1:5000, WB:1:500-1:2000
Western Blot (WB)ELISA:1:2000-1:5000, WB:1:500-1:2000

Immunogen: Synthetic peptide of Human HIF1A

Antigen Species: Human

Alternative Names

ARNT interacting protein; ARNT-interacting protein; Basic helix loop helix PAS protein MOP1; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF 1alpha; HIF-1-alpha; HIF-1alpha; HIF-alpha; HIF1 A; HIF1 Alpha; HIF1; HIF1-alpha; HIF1A; HIF1A_HUMAN; hifla; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3 ; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Hypoxia inducible factor 1; alpha subunit (basic helix loop helix transcription factor); Hypoxia inducible factor1alpha; Hypoxia-inducible factor 1-alpha; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; MOP1; PAS domain-containing protein 8; PASD 8; PASD8

What species does this HIF1A antibody react with?
This antibody has been validated for reactivity against Human, Mouse, Rat. Cross-reactivity is based on the immunogen sequence and may vary by sample type.
What applications has this antibody been validated for?
Validated applications include: ELISA, Western Blot (WB). Each application was confirmed with appropriate controls. Contact technical support for applications not listed.
What is the recommended dilution?
ELISA:1:2000-1:5000, WB:1:500-1:2000. Optimal concentrations depend on sample type, detection system, and antigen abundance. Titration with positive and negative controls is recommended.
How was this antibody produced?
This polyclonal IgG antibody was raised in Rabbit and purified by affinity chromatography using Protein G. It provides high specificity and minimal cross-reactivity in standard immunoassay applications.
What are the storage requirements?
Store at −20°C for long-term. Avoid repeated freeze-thaw cycles. Short-term working aliquots may be stored at 4°C. This product is supplied in a stabilized buffer containing BSA and sodium azide.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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